シナプス伝送中のミトコンドリア伝導量の長期的活性化
E A Jonas1, J Buchanan, L K Kaczmarek
1Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA.
Science (New York, N.Y.)
|November 13, 1999
まとめ
科学者は,無傷のイカのシナプス前端にミトコンドリアイオンチャネル活動を記録しました. シナプス刺激により,ミトコンドリア膜の伝導性が著しく増加し,ミトコンドリアの調節のための新しいメカニズムが明らかになりました.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
- バイオフィジックス 生物物理学
背景:
- ミトコンドリアのイオンチャネルは知られているが,無傷の細胞で研究するのは難しい.
- 以前の研究では,細胞のミトコンドリア内のイオン輸送の直接記録がありませんでした.
研究 の 目的:
- 健全な細胞のミトコンドリアのイオンチャネル活動を記録し,特徴づけること.
- 神経細胞活動中のミトコンドリアイオン輸送の変化を調査する.
主な方法:
- スクイードのプレシナプス端末に修正されたパッチクランプ技術を使用しました.
- ミトコンドリアが電子顕微鏡で標的器官であることを確認した.
主要な成果:
- 無傷のプレシナプス端末のミトコンドリアのイオンチャネル活動を記録しました.
- シナプス刺激後のミトコンドリア膜伝導率の有意な,遅延した,持続的な増加 (最大60倍) が観察されました.
- 誘導力が刺激が止まった後も上昇し,数十秒で回復したことに注意した.
結論:
- ミトコンドリアのイオンチャネル活動を in situ で記録する可能性を実証した.
- 神経細胞の活動とミトコンドリアのイオン輸送のダイナミックな変化の間のリンクを確立しました.
- シナプス伝達におけるミトコンドリア機能を理解するための新しい道を開いた.
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