関連する実験動画

Updated: Jul 12, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

RCAF複合体は,DNA複製と修復の間にクロマチンの組立を媒介する

J K Tyler1, C R Adams, S R Chen

  • 1Department of Biology and Center for Molecular Genetics, University of California at San Diego, La Jolla 92093-0347, USA.

Nature
|December 11, 1999
PubMed
まとめ

研究者らは,新しいタンパク質複合体,複製結合組立因子 (RCAF) を特定し,複製されたDNA上で核細胞を作るために決定的な役割を果たした. ASF1とヒストンH3/H4を含むRCAFは,細胞サイクル進行とDNA修復に不可欠です.

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Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter06:59

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

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Base Excision Repair01:54

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Base Excision Repair01:54

Base Excision Repair

One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
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