集団ベースの分子疫学によるMycobacterium tuberculosisのW変異の発生の特定
1Public Health Research Institute Tuberculosis Center, Department of Microbiology, New York University School of Medicine, NY 10016, USA.
JAMA
|December 28, 1999
まとめ
分子型検査により,ニュージャージー州でこれまでに検出されなかったMycobacterium tuberculosisの発生が確認されました. このアプローチは,DNAの指紋を用いて,明確なWファミリー株と患者の人口を明らかにし,ターゲット化された公衆衛生の介入を支援しました.
科学分野:
- エピデミオロジー エピデミオロジー
- 分子生物学は分子生物学である.
- 公衆衛生は公衆衛生である.
背景:
- 従来の監視だけでは,Mycobacterium tuberculosisのアウトブレイクを検出するには不十分です.
- 分子型は,結核の伝播を特定するためのより敏感な方法を提供します.
- ニューヨーク市 W 株の M. tuberculosis の変種は以前,ニュージャージー州で確認されていた.
研究 の 目的:
- ニュージャージー州におけるM.結核菌株のW族の拡散を特徴づける.
- 菌株の拡散を記述するために,分子タイプ化と監視データを活用する.
- Wファミリーの菌株に関連した特定の人口および地理的なパターンを特定する.
主な方法:
- 1996年1月から1998年9月までの結核症例を分析した人口ベースの横断的な研究.
- 分子型タイプ技術には,IS6110のDNA指紋,PGRS型タイプ,スポリゴタイプ,VNTR分析が含まれていました.
- 菌株の特徴は,従来の監視データで補完された.
主要な成果:
- 1207種のうち68種 (6%) がW族に属していた. IS6110パターンに基づいて2つの異なるグループ (AとB) が特定されました.
- グループAの株 (43人の患者) は密接に関連しており,グループBの株 (25人の患者) はより多様でした.
- グループAの患者は主に米国生まれで,黒人であり,HIV陽性であり,ニュージャージー州北東部の都市部に住んでいた.
結論:
- 監視データと組み合わせた複数の分子技術により,これまでに検出されなかった結核の発生を成功裏に特定しました.
- この疫病は,W族の系統遺伝子の特定の枝に関係していた.
- 分子菌株タイプ化は,疫病発生調査を開始するための積極的な戦略を提供し,従来の監視を強化します.
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