ゴルギの膜は,ミトーシス過程でERに吸収され,そこから再現します
K J Zaal1, C L Smith, R S Polishchuk
1Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Istitutes of Health, Bethesda, Maryland 20892-5430, USA.
Cell
|December 28, 1999
まとめ
ゴルギ装置は,ミトーシス過程でERに分散し,ERリサイクル経路を通じて改革し,ゴルギ遺伝と起源の以前のモデルに異議を唱えます.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- オルガネルダイナミクス オルガネルダイナミクス
背景:
- ゴルギ装置は,タンパク質の改変と輸送に不可欠です.
- 細胞分裂 (ミトーシス) 中のその振る舞いは議論されている.
- ゴルギのダイナミクスを理解することは,細胞分裂と遺伝の鍵です.
研究 の 目的:
- インターフェーズ細胞におけるゴルギタンパク質のリサイクルを調査する.
- ミトーシス中のゴルギ構造の変化を監視するために.
- ミトーシス後のゴルジのリフォーメーションのメカニズムを解明する.
主な方法:
- 生体細胞における定量画像と光漂白.
- GFPでタグ付けされたゴルギタンパク質を追跡する.
- 免疫電子顕微鏡 (免疫電子顕微鏡) とは
主要な成果:
- ゴルギのタンパク質は,インターフェーズで1.5時間ごとにリサイクルされます.
- ミトーシス過程で,ゴルギのタンパク質はERに再分配されます.
- 異なったミトのゴルギ小胞は検出されませんでしたが,成分はERと融合しています.
- ゴルギ・リフォーメーション・ポスト・ミトーシスは,ERの輸出を必要とする.
結論:
- ゴルギはミトーシス過程でERに分散する.
- ゴルギ・リフォームは,ERの輸出とリサイクル経路に依存しています.
- これは,ゴルギの生物発生と遺伝に関する新しいパラダイムを定義しています.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
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One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
01:43The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
01:43The Endoplasmic Reticulum
The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...