胚分裂による霊長類の子孫のクローン繁殖

A W Chan¹, T Dominko, C M Luetjens

¹Oregon Regional Primate Research Center, Beaverton, OR 97006, USA.

Science (New York, N.Y.)

|January 15, 2000

まとめ

科学者は,胚分裂を使用して,遺伝的に同一の非ヒト霊長類を作成しました. この画期的な発見は,ヒトの病気を研究するための理想的なモデルを提供し,生きたクローンの子孫の可能性を示しています.

科学分野:

生殖生物学生殖生物学
霊長類の遺伝学
発達生物学発達生物学とは

背景:

現在のクローニング方法は,核と細胞プラズマのコンポーネントで同一の霊長類を生成しません.
同一型の霊長類のモデルは,人間の病気の調査に不可欠です.

研究の目的:

ブラストメア分離と再集約を使用して,遺伝子的に同一の非ヒト霊長類の胚を生成する.
これらのクローン胚の生存能力と発達可能性を評価する.

主な方法:

分裂段階の rhesus 胚は分割され,ブラストメアは再集約され,複数の遺伝的に同一の胚を生成しました.
107個の rhesus 胚から合計 368 匹の倍数が生成されました.
13個の胚移植が行われ,妊娠率は,インビトロ受精対照と比較して高かった.

主要な成果:

遺伝的に同一の非人間霊長類の胚は,双子やより大きなセットとして成功裏に生産されました.
胚移植から4人の妊娠が確認された.
4分の1の胚から健康なクローンメスレサス猿,テトラの誕生が達成されました.

結論:

胚の分裂と再集積は,遺伝子的に同一の非ヒト霊長類を生産するための有効な方法である.
この技術により,生きた子孫が生み出され,病気の研究に貴重なモデルとなる.
テトラのクローニングの成功は,将来の研究のためにこのアプローチの可能性を検証しています.

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