イーストのプリオン種のバリアル種の分子基盤
A Santoso1, P Chien, L Z Osherovich
1Department of Cellular & Molecular, University of California, San Francisco 94143-0450, USA.
Cell
|February 5, 2000
まとめ
酵母プリオンタンパク質Sup35pの遺伝は種間保たれ,種間誘導を防ぐバリアがある. この障壁は特定の集積エピトープによって媒介され,複数の酵母プリオン状態の独立した伝播を可能にします.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- プリオン生物学 プリオン生物学
背景:
- 酵母[PSI+]因子であるプリオンは,自己組み立てのSup35p集積物によって増殖する.
- プリオンメカニズムは,様々な生物の遺伝性特性に不可欠です.
研究 の 目的:
- イースト種におけるSup35pプリオン機能の保存を調査する.
- イーストのプリオン伝播における種障壁の分子基礎を解明する.
- 種間プリオン誘導を可能にするメカニズムを特定する.
主な方法:
- 遠い親戚の酵母におけるSup35pプリオン機能の比較分析.
- プリオン誘導と種のバリアを研究するためのインビトロポリメリゼーションアッセイ.
- バリア越えに関与するドメインをマッピングするためのキメリックタンパク質分析.
主要な成果:
- Sup35pプリオン機能と遺伝は,様々な酵母種で保存されています.
- 種のバリアは,異なる酵母Sup35p変種間のプリオン誘導を阻害し, in vitroでも観察されています.
- 特定の短い領域が特定され,外来Sup35pが種障壁を克服することを可能にしました.
- 複数のプリオンベースの遺伝状態は,単一の細胞内で独立に伝播することができる.
結論:
- 酵母プリオンにおける種障壁は,定義されたエピトープによって媒介されるアミロイド集積表面での特定の相互作用から生じる.
- 新しい酵母プリオン領域が特定され,プリオン多様性の理解に貢献しました.
- これらの発見は,異なるプリオン状態が酵母細胞内で独立して共存し,拡散できることを示している.
関連する概念動画
01:28Yeast Signaling
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
01:17Understanding Species and Reproductive Barriers
A species is a group of organisms that interbreed and produce fertile offspring. Typically, individuals of the same species appear similar and share common characteristics due to their highly similar genomes. However, not all organisms that look alike are members of the same species. Various mechanisms keep most species discrete. While some mechanisms prevent reproductive behavior and fertilization (pre-zygotic isolation), others prevent the production of fertile offspring after mating has...
01:57Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
01:33Overview of Secretory Vesicles
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...