遺伝子発現プロファイリングによって識別される分散型大B細胞リンパ腫の異なるタイプ
A A Alizadeh1, M B Eisen, R E Davis
1Department of Biochemistry, Stanford University School of Medicine, California 94305, USA.
Nature
|February 17, 2000
まとめ
分散型大B細胞リンパ腫 (DLBCL) は分子異質性を示しています. 遺伝子発現プロファイリングにより,生殖中心のB細胞型のDLBCLと活性化されたB細胞型のDLBCLの2つのサブタイプが特定され,生存結果は異なる.
科学分野:
- 腫瘍学 腫瘍学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- 拡散型大B細胞リンパ腫 (DLBCL) は,最も一般的なノンホジキンリンパ腫です.
- DLBCL患者の臨床結果は高度に変動し,基礎となる分子差異を示唆しています.
- 以前の分類は,この異質性を完全に捉えることができなかった.
研究 の 目的:
- DLBCL腫瘍の分子異質性を調査する.
- 遺伝子発現プロファイルに基づいて,DLBCLの異なる分子サブタイプを識別する.
- 分子サブタイプと臨床結果,特に患者の生存率を相関させる.
主な方法:
- DLBCL腫瘍の系統的な遺伝子発現プロファイリングのためにDNAマイクロアレイを使用しました.
- 遺伝子発現パターンを分析し,異なる分子シグネチャを特定しました.
- 特定された分子サブタイプと患者の生存データとの相関関係.
主要な成果:
- DLBCLの2つの異なる分子サブタイプを特定しました:生殖中心のB細胞型のDLBCLと活性化されたB細胞型のDLBCLです.
- ゲルミナルセンターB細胞のようなDLBCL腫瘍は,ゲルミナルセンターB細胞の特徴的な遺伝子を発現させた.
- アクティベートされたB細胞型のDLBCL腫瘍は,B細胞の活性化と関連した遺伝子をインビトロで発現させた.
- ゲルミナルセンターB細胞類似DLBCLを有する患者は,活性化されたB細胞類似DLBCLを有する患者と比較して,著しく高い全生存率を示した.
結論:
- 遺伝子発現プロファイリングは,DLBCL内の有意な分子異質性を明らかにします.
- DLBCLの2つの臨床的に重要なサブタイプ,生殖中心のB細胞様と活性化されたB細胞様が特定されています.
- 遺伝子発現に基づく分子分類は,患者の生存率を予測し,異なるがんサブタイプを特定することができます.
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