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関連する概念動画

Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

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The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
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Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

1.2K
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
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Operon Model01:23

Operon Model

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The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
976
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.1K
Operons02:09

Operons

53.8K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
53.8K
Riboswitches01:56

Riboswitches

9.4K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Light-Controlled Fermentations for Microbial Chemical and Protein Production
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代替モジュール型ポリケチド合成酵素発現制御 マクロラクトン構造 マクロラクトン構造

Y Xue1, D H Sherman

  • 1Department of Microbiology and Biological Process Technology Institute, University of Minnesota, Minneapolis 55455, USA.

Nature
|February 17, 2000
PubMed
まとめ
この要約は機械生成です。

モジュラーポリケチド合成酵素 (PKS) の代替表現により,多様なマクロラクトン構造が生成されます. この研究では,PikAIV PKSを改変することで,異なるナルボノリドおよび10-デオキシメチノリド製品が生成される方法が明らかにされています.

さらに関連する動画

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 自然製品 化学 化学

背景:

  • モジュラーポリケチド合成酵素 (PKS) は,構造的に多様なポリケチドを合成する大型酵素で,様々な生物学的活動を持っています.
  • PKSモジュール内の酵素ドメインの配置は,最終的なポリケチド製品構造を決定する.
  • ポリケチドは,医学や産業で応用できる重要な天然製品です.

研究 の 目的:

  • ポリケチド生物合成に対するピクロミシンPKS (PikAIV) の代替表現の影響を調査する.
  • ポリケチドの自然産物の構造的多様性につながる可能性について,PKS発現の変異を調査する.
  • モジュール式PKSの構造-機能関係に関する洞察を提供するためです.

主な方法:

  • Streptomyces venezuelae.で全長およびアミノ端末切断されたPikaIVの代替表現を調査しました.
  • 生化学的および遺伝的アプローチを使用して,結果のマクロラクトン構造を分析しました.
  • 製品であるナルボノリドおよび10-デオキシメチノリドの特徴を示した.

主要な成果:

  • 完全長さのPikAIVの発現により,14個のマクロラクトンナルボノリドが産生されました.
  • 切断されたPikAIVの発現は,凝縮サイクルをスキップし,12個のマクロラクトン10-デオキシメチノリドを生成しました.
  • 代替PKS発現がマクロラクトンのリングサイズに直接影響することを示した.

結論:

  • モジュール式PKSの代替表現は,ポリケチド性天然製品の構造的多様性を生み出す戦略を提供します.
  • この発見は,PKSの組み立てラインメカニズムと製品特異性に関する理解を深める.
  • ポリケチドを標的とした合成生物学と薬剤発見の取り組みのための新しいツールを提供します.