タンパク質キナーゼ PINOIDによってオクシン反応の調節

S K Christensen¹, N Dagenais, J Chory

¹The Salk Institute for Biological Studies, La Jolla, California 92037, USA.

Cell

|February 29, 2000

まとめ

PINOID (PID) 遺伝子の変異は,アラビドプシスの発達を妨げ,臓器形成に影響する. PIDは,植物の成長と発達に不可欠なオクシンシグナル伝達を否定的に調節する.

科学分野:

植物生物学植物生物学
分子遺伝学分子遺伝学
発達生物学発達生物学とは

背景:

アラビドプシス・タリアナのPINOID (PID) 遺伝子の変異は,オクシン輸送阻害剤によって引き起こされるものと同様の発達障害を引き起こす.
これらの欠陥には,コチレドン,花,および花器官の形成が損なわれ,植物発達の過程でPIDの役割が示唆される.

研究の目的:

PID遺伝子をクローンし,植物の発達におけるその役割を調査する.
アクシンシグナル伝達経路に関連してPIDの機能を明らかにする.

主な方法:

PINOID (PID) の遺伝子クローン.
胚形成と花の発達中のPID発現パターンの分析.
変化したPID発現 (変異と構成発現) を有する植物のフェノタイプ分析.

主要な成果:

PID遺伝子はクローンされ,胚と花原植物で一時的に発現することが判明しました.
構成的なPID発現は,オクシン無感性の変異体と同様の芽と根の両方のフェノタイプをもたらしました.
PIDはオクシンシグナル伝達の負の調節剤として作用する.

結論:

PIDは,原始の発達を促進する上で特別な役割を果たします.
PIDはオクシンシグナル伝達を否定的に調節し,植物全体の発達に影響を与えます.

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