S受容体キナーゼは,Brassica stigmaにおける自己不適合性を決定する
T Takasaki1, K Hatakeyama, G Suzuki
1Research Institute of Seed Production Co., Ltd., Sendai, Japan. taka@kobe-u.ac.jp
Nature
|March 8, 2000
まとめ
ブラシカの自己不互換性は,S受容体キナーゼ (SRK) とSロカスグリコタンパク質 (SLG) の遺伝子に依存しています. SRKだけで花粉の拒絶特異性を決定し,SLGは自己相容れない反応を強化する.
科学分野:
- 植物の生殖生物学 植物の生殖生物学
- 分子遺伝学 分子遺伝学
- 植物育種 植物育種
背景:
- 自己不適合性 (SI) は,自己受精を防ぐ,ブラシカの重要な生殖障壁である.
- SIを制御するSロカスには,S受容体キナーゼ (SRK) とSロカスグリコプロテイン (SLG) の2つの重要な遺伝子が含まれています.
- これらの遺伝子は,スティグマパピラー細胞で発現し,花粉の認識と拒絶を媒介すると考えられています.
研究 の 目的:
- ブラシカの自己不互換性におけるSRKとSLGの個々の役割を明らかにする.
- SRKまたはSLGが単独で自己花粉の拒絶を引き起こすかどうかを判断する.
- 自己相容れない反応に対するSRKとSLGの相乗効果を調査する.
主な方法:
- SRK28とSLG28トランスゲンを持つ自己相容れないBrassica rapaの遺伝子変換.
- SRK28単独,SLG28単独,または両方を発現するトランスジェニック植物における花粉拒絶の分析.
- 改変したブラシカ植物における自己相容れない反応のフェノタイプ評価.
主要な成果:
- SRK28トランスゲンの発現だけで,自己 (S28) 花粉を拒絶する能力を授与しました.
- SLG28のトランスゲン発現だけでは,自己花粉の拒絶を引き起こさなかった.
- SRK28によって媒介された花粉拒絶能力は,SLG28.2の共発現によって著しく強化されました.
結論:
- SRKだけでは,Brassicaの自己不適合性におけるスティグマのSハプロタイプ特異性を決定するのに十分です.
- SLGは調節器として機能し,自己相容れない反応の完全な顕現を高めます.
- これらの発見は,ブラシカの生殖孤立におけるSRKとSLGの異なる,しかし協力的な役割を明確にします.
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