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細胞表面のエストロゲン受容体は,ヒト内皮細胞におけるカルシウム依存の酸化窒素の放出を媒介する
G B Stefano1, V Prevot, J C Beauvillain
1Neuroscience Research Institute, State University of New York (SUNY) at Old Westbury, NY 11568-0210, USA.
Circulation
|April 4, 2000
まとめ
エストロゲンは,ヒト内皮細胞からの酸化窒素 (NO) の放出を迅速に促進します. これは細胞表面のエストロゲン受容体を通じて発生し,細胞内カルシウムを活性化し,エストロゲンを説明する可能性がある.
科学分野:
- エンドクリノロジー エンドクリノロジー
- 心血管生理学 心血管の生理学
- 分子生物学は分子生物学である.
背景:
- エストロゲン置換療法は,閉経後の女性の心血管イベントを軽減することができます.
- エストロゲンの心血管への効果の背後にある正確なメカニズムは完全に理解されていません.
- 酸化窒素 (NO) は,血管を保護するエンドセリウム由来の重要な因子です.
研究 の 目的:
- ヒト内皮細胞からのNO放出に対する生理学的エストロゲンレベルの急性効果を調査する.
- エストロゲンが細胞表面受容体を通してNOの放出を刺激するかどうかを判断する.
- エストロゲン媒介のNO放出に関与するシグナル伝達経路を解明する.
主な方法:
- アンペアメトリックプローブを使用してNO放出のリアルタイム測定.
- 人間の胸内動脈と動脈内皮細胞を17β-エストラジオールに曝露する.
- 17β-エストラディオールと牛の血清アルブミン結合を用いて,受容体の関与を評価する.
- タモキシフェンを用いてエストロゲン受容体を抑制する.
- 細胞内カルシウムトランジントを二重放出マイクロフルオロメトリーで測定する.
主要な成果:
- 17β-エストラディオールは,濃度に依存した方法で,内皮細胞からNOの放出を迅速に刺激しました.
- エストロゲンは,結合されたエストラディオールとタモキシフェンの対抗性によって示されるように,細胞表面受容体を通して作用することを証拠が示唆しています.
- エストロゲン刺激によるNO放出は,細胞内カルシウムの初期上昇に依存する.
結論:
- 生理学的エストロゲン用量は,ヒト内皮細胞からの酸化窒素 (NO) の放出を急激に刺激する.
- この刺激は,細胞表面のエストロゲン受容体によって媒介されます.
- この経路は,細胞内カルシウムシグナル伝達の活性化を伴う.
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