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Taste Preference Assay for Adult Drosophila
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T2Rは苦い味の受容体として機能する
J Chandrashekar1, K L Mueller, M A Hoon
1Howard Hughes Medical Institute and Department of Biology, University of California, San Diego, La Jolla 92093, USA.
Cell
|April 13, 2000
まとめ
哺乳類の苦い味覚受容体 (T2Rs) を特定し,機能的に特徴づけました. 特定のT2Rは,サイクロヘキシミドのような苦い化合物を検出することが示され,動物が有毒物質を避ける方法を説明しました.
科学分野:
- 分子生物学は分子生物学である.
- 感覚神経科学は,感覚神経科学である.
- 遺伝学 遺伝学とは
背景:
- 苦い味の知覚は,毒素を摂取することに対する重要な防御メカニズムです.
- 哺乳類の味覚受容体,特にT2Rは,苦い化合物の検出に関与しています.
研究 の 目的:
- 特定の哺乳類の味覚受容体 (T2Rs) を機能的に特徴付ける.
- T2Rsが苦い味の受容体として機能することを示すために.
主な方法:
- 受容体の機能をテストするために使用される異質表現システム.
- 変異したサイクロヘキシミド検出のマウス株の分析.
- マウスT2R (mT2R-5) を昆虫の細胞に発現させ,Gタンパク質の活性化を研究する.
主要な成果:
- 特定のT2Rs (mT2R-5,hT2R-4,mT2R-8) は,苦い味 (サイクロヘキシミド,デナトニウム,6-n-プロピル-2-チオウラシル) に反応した.
- マウスにおけるmT2R-5の遺伝的変異は,変化したサイクロヘキシミド検出と相関する.
- mT2R-5の活性化により,特定の味覚に依存するgustducin.cinの活性化が生じました.
結論:
- 特定のT2Rは苦い味の受容体として機能し,有毒化合物の検出を媒介する.
- 味覚細胞で表現されるT2Rの多様なレパートリーは,様々な苦い物質の知覚を説明します.
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