N-カデリンは,胚の左-右非対称性の確立に関与する細胞粘着分子です

M I García-Castro¹, E Vielmetter, M Bronner-Fraser

¹Division of Biology 139-74, California Institute of Technology, Pasadena, CA 91125, USA. mig1000@caltech.edu

Science (New York, N.Y.)

|May 12, 2000

まとめ

細胞粘着分子であるN-カデリンは,鶏の胚の左-右軸の形成に不可欠です. その非対称的な発現と, gastrulation 中の機能は,正しい臓器の発達と心臓の循環に不可欠です.

科学分野:

発達生物学発達生物学とは
細胞の粘着性細胞の粘着性
胚形成とは,胚の形成である.

背景:

脊椎動物の胚は双方向対称性を示すが,多くの臓器は非対称的に発達する.
左から右の軸の決定の基礎となる分子機構は複雑で,完全に理解されていません.

研究の目的:

脊椎動物の発達初期における左右非対称性の確立におけるN-カデリンの役割を調査する.
N-カデリンが非対称性経路の初期の構成要素であるかどうかを判断する.

主な方法:

ガストルーレーション中の鶏の胚におけるN-カデリン発現を研究した.
ブロックされたN-カデリンの機能は,心臓のループと遺伝子発現に及ぼす影響を観察する.

主要な成果:

N-カデリンは,鶏の胚形成中にアシンメトリックに発現した最も初期のタンパク質の1つです.
N-カデリンのランダム化された心臓ループをブロックし,スナイルとPitx2の発現を変化させた.
ノダルとレフティの表現は変化せず,平行経路を示唆した.

結論:

N-カデリン媒介の細胞結合は,左-右非対称性を確立するために不可欠です.
これらの発見は,臓器発達の並列メカニズムを含む可能性のある経路におけるN-カデリンの初期の役割を強調しています.

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