Dishevelledは,Xenopus gastrulationの間に細胞の極性を制御しています
J B Wallingford1, B A Rowning, K M Vogeli
1Department of Molecular and Cell Biology, University of California, Berkeley 94720-3204, USA.
Nature
|May 16, 2000
まとめ
Xenopus Dishevelled (Xdsh) は,胚の発達に不可欠な細胞の極性を制御しています. この研究は,Xdshが,脊椎動物の胚形成に不可欠な細胞の極性を維持することによって,胃化の間に細胞の動きを調節することを明らかにしています.
科学分野:
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
背景:
- 細胞の動きは胚の発達に不可欠ですが,脊椎動物における細胞の動きの調節は依然として十分に理解されていません.
- Xenopus Dishevelled (Xdsh) の阻害は胃細胞の動きを妨げますが,その背後にあるメカニズムは不明です.
- 細胞の極性におけるDishevelledの役割はDrosophilaで知られているが,脊椎動物の細胞の極性におけるその機能は未調査である.
研究 の 目的:
- Xenopus Dishevelled (Xdsh) が gastrulation 中の細胞の動きを調節するメカニズムを調査する.
- Xdshが脊椎動物胚の細胞極性を制御するかどうかを判断する.
- 収束延伸運動における極化細胞行動の役割を明らかにする.
主な方法:
- タイムラップコンフォカル顕微鏡で,Xenopus胚の細胞の行動を観察する.
- 収束拡張に影響するXdsh変異の分析.
- 背面メソダーマ細胞におけるXdshの局所化を調査する.
主要な成果:
- Xdsh機能の欠如は,細胞の極性における欠陥につながり,収束拡張の失敗を引き起こします.
- 特定のXdsh変異は,Drosophila Dishevelled変異と相関し,平面細胞の極性に影響を与える.
- Xdshは,正常な背面メソダーマ細胞の細胞膜に局所され,極性におけるその役割をサポートします.
結論:
- 分極化された細胞の行動は,脊椎動物 gastrulation 中の収束的な拡張に不可欠です.
- Vertebrate Dishevelled (Xdsh) は,細胞の極性を制御しています.
- ドロソフィラの平らな細胞の極性経路に似たシグナリングカスケードは,脊椎動物の胃流のために必要である可能性があります.
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