関連する実験動画

Updated: Jan 12, 2026

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Published on: January 31, 2020

JNKはエフェクターT細胞の機能には必要ですが,T細胞の活性化には必要ありません

C Dong¹, D D Yang, C Tournier

¹Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA.

|May 16, 2000

まとめ

c-Junアミノ端末キナーゼ (JNK) 経路は,T細胞の初期活性化ではなく,T細胞の分化に不可欠である. JNKシグナル伝達が欠けているマウスは,IL-2の産生と増殖が強化されたが,エフェクターサイトカイン生成が低下した.

科学分野:

免疫学免疫学とは
分子生物学は分子生物学である.
細胞シグナリング細胞シグナリング

背景:

インターリューキン2 (IL-2) 生産はT細胞活性化の重要な指標である.
c-Junアミノ端末キナーゼ (JNK) は,AP-1転写因子活性とIL-2発現に関与するMAPキナーゼである.
以前の研究では,JNK1/JNK2欠乏がT細胞サブセットの分化に影響を及ぼすが,ネイブT細胞のIL-2生成には影響しないことが示された.

研究の目的:

ネイブT細胞活性化とIL-2発現におけるJNK信号伝達経路の重要な役割を調査する.
JNKシグナル伝達がIL-2生成とT細胞増殖に不可欠であるかどうかを判断する.
エフェクターT細胞のサイトカイン生成と分化におけるJNKの必要性を明らかにする.

主な方法:

JNKタンパク質が全く存在しない3つの新しいマウスモデルを使用し,外周T細胞のシグナル伝達も行いました.
野生型対照群と比較して,JNKシグナル伝達が欠けているT細胞のIL-2生成および増殖能力を評価した.
エフェクターT細胞サイトカインの生成のためのJNKの要件を評価した.

主要な成果:

JNKシグナル伝達が完全に欠けていたT細胞は,野生型T細胞と比較して,予想外にも高いIL-2生成と増殖を示した.
JNKシグナリングは,ナイブT細胞活性化とIL-2発現に欠かせないことが判明しました.
分化T細胞によるエフェクターサイトカインの生成は,JNKシグナル伝達に依存していた.

結論:

JNKシグナリングは,ナイブT細胞の初期活性化やIL-2生成には必要ありません.
JNKは,T細胞を特定のサブセットとエフェクタ機能に分離する上で重要な役割を果たします.
JNK経路はT細胞の分化に不可欠ですが,T細胞の活性化には欠かせません.

さらに関連する動画

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

関連する概念動画

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Cells of the Innate Immune Response

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026

First Observation of the B[over ¯]_{s}^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay and Evidence for the B[over ¯]^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay.

Physical review letters·2026

関連記事をすべて見る