転写活性TBPのTAF含有型およびTAF独立型は,体内に存在する
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Science (New York, N.Y.)
|May 20, 2000
まとめ
酵母遺伝子の転写は,TBP結合タンパク質 (TBP) を2つの形態で含む:TBP関連因子 (TAF) があるか否か. 熱ショックは,TAFを含むTFIID複合体を好み,差異的な遺伝子活性化を説明します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- イースト遺伝学 イースト遺伝学
背景:
- 酵母における転写活性は,一般的な転写因子のプロモーター占有量と関連しています.
- TATA結合タンパク質 (TBP) と関連因子 (TAF) の酵母転写における役割については,さらなる解明が必要である.
研究 の 目的:
- イーストの転写活動に関与するTBPの異なる形態を調査する.
- 熱ショックがTBPを含む複合体の利用にどのように影響するかを決定する.
- 特定の酵母遺伝子のTFIID依存転写の基礎を探求する.
主な方法:
- 酵母におけるTBP,TFIIA,TFIIB,TAFによるプロモーター占有量の分析.
- TBPのフォーム利用の比較,通常の条件と熱ショック条件下.
主要な成果:
- イーストの転写活動は,TBP,TFIIA,TFIIBの占有率と相関しているが,TAFはそうではない.
- TBPは,TFIID (TAFsを含む) とTBP単独または他の複合体 (TAFsなし) の少なくとも2つの活性形態が存在します.
- 熱ショック治療は,TFIID複合体の相対的な利用率を増加させます.
結論:
- TFIIDとTAFのないTBPを含むTBPの明確な形態は,酵母で転写的に活性化されています.
- これらのTBP形態の異なるプロモーター特異的関連は,遺伝子転写に影響を与える.
- 熱ショックはTBP形態のバランスを変化させ,TFIIDを好み,この複合体の遺伝子特異的な要求を潜在的に説明します.
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