関連する実験動画

Updated: Aug 10, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

転写因子IIHによるATP依存プロモーター溶解のメカニズム

T K Kim1, R H Ebright, D Reinberg

  • 1Howard Hughes Medical Institute, Division of Nucleic Acids Enzymology, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.

Science (New York, N.Y.)
|May 29, 2000
PubMed
まとめ

転写因子IIH (TFIIH) は,そのERCC3サブユニットを使用して,転写を開始するためにDNAプロモーターを溶かします. TFIIHは,従来のヘリケアゼではなく,モルキュラーレンチのように作用し,下流のDNAを回転させトルクを生成し,介入DNAを溶かします.

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Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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