ホメオドメインのタンパク質コードは,ベントラルニューラルチューブの原始細胞のアイデンティティとニューロンの運命を指定します
J Briscoe1, A Pierani, T M Jessell
1Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA.
Cell
|June 1, 2000
まとめ
ソニック・ヘッジホッグ (Shh) 信号パターンは,腹部神経管の異なる神経系のクラスである. Shhによって調節される転写因子は,特定のニューロンタイプを生成する原始ドメインを定義します.
科学分野:
- 発達生物学 発達生物学とは
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
背景:
- 腹部神経管は,異なる種類のニューロンを生成する.
- ソニック・ヘッジホッグ (Shh) 信号グラデーションパターン 神経発達.
- ホメオドメインの転写因子は,Shh依存神経パターンを媒介する.
研究 の 目的:
- Shh媒介神経パターニングにおけるホメオドメイン転写因子の役割を明らかにする.
- Shhシグナル伝達が異なる祖先ドメインを確立する方法を定義する.
- ニューロンのアイデンティティを指定する組み合わせコードを理解する.
主な方法:
- Shh信号伝達経路の分析.
- ホメオドメイン転写因子の発現プロファイリング (クラスIとII).
- 転写因子間の交差抑制相互作用の調査.
主要な成果:
- Shhのアクティビティ・グラディエントは5つの祖先ドメインを定義する.
- クラスIの転写因子はShh抑制され,クラスIIの因子はShhを必要とする.
- Nkx6.1,Nkx2.2およびIrx3の組み合わせ表現は,3つの内側神経細胞のクラスを指定します.
結論:
- ホメオドメインの転写因子は,Shh依存神経パターニングの仲介者として作用する.
- 転写因子クラスの間の相互作用は,前身ドメインを精製する.
- ベントラルニューラルチューブの特定のニューロンのアイデンティティの組み合わせ表現コード.
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