人間の動脈硬化性プラークにおける内皮細胞アポトーシスと血流方向の関係
1Institut National de la Santé et de la Recherche Médicale, INSERM U541, Institut Fédératif de Recherche "Circulation," Hôpital Lariboisière, Paris, France.
Circulation
|June 1, 2000
まとめ
人間の動脈硬化症における内皮細胞アポトシスは,主に血流と剪定ストレスが低いプラークの下流で発生します. この発見は,シアストレスがプラークの侵食と血栓形成に影響することを示唆しています.
科学分野:
- 心血管生物学 心血管生物学
- 内皮細胞生物学 内皮細胞生物学
- 動脈硬化症の研究研究
背景:
- 血流のダイナミクスは,内皮細胞アポトーシスに重大な影響を及ぼします.
- 人間の動脈硬化症における内皮細胞アポトーシスの特定の役割と,血流との相関性は,依然としてほとんど特徴づけられていない.
研究 の 目的:
- 人間の動脈硬化プラークにおける内皮細胞アポトーシスの発生と局所化を調査する.
- 血流の特徴,特にシアストレスの関係と,動脈硬化症における内皮細胞アポトーシスの関係を決定する.
主な方法:
- エンドアテレクトミーで得られた42人の人の動脈動脈動脈硬化性プラークの分析.
- CD31,Ki-67およびスプライシングファクター抗体を用いた免疫ヒスト化学分析.
- 末端のdUTPニークエンドラベリングとリガースアッセイを用いたアポトーシスのインシット検出.
主要な成果:
- エンドセリウム細胞のアポトシスは,処理された13つのプラークのうち8つで観察されました.
- アポトーシスは主にプレックの下流地域 (18.8%) で,上流地域 (2.7%) に比べて発生しました.
- アポプトティック内皮細胞は,プラークの微小容器ではめったに検出されませんでした.
結論:
- ローカル・シーア・ストレスは,体内では,光線内皮細胞アポトシスに有意な影響を及ぼします.
- 切断ストレスは,プラーク侵食と血栓形成の進行における重要な要因である可能性があります.
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