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ヒトの血清パラオクソナゼ (PON1) QとRは,ヒトの冠動脈および動脈動脈動脈硬化病変における脂質ペロキシドを選択的に減少させます:PON1エステラゼとペロキシダゼのような活動:PON1エステラゼとペロキシダゼのような活動
1Lipid Research Laboratory, Technion Faculty of Medicine, the Rappaport Family Institute for Research in the Medical Sciences, Rambam Medical Center, Haifa, Israel. aviram@tx.technion.ac.il
Circulation
|June 1, 2000
まとめ
ヒトの血清パラオキソナーゼ (PON1) イソフォームQとRは,動脈硬化性損傷における脂質過酸化を減少させます. この水解は PON1 を示します.
科学分野:
- バイオケミストリー バイオケミストリー
- 心血管科学 心血管科学
- 酵素学 酵素学とは
背景:
- 人間の血清パラオキソナーゼ (PON1) は,位置192 (Q/R) と位置55 (M/L) でポリモルフィズムを示しています.
- PON1は低密度脂質タンパク質 (LDL) の酸化に対する保護的役割を果たし,この過程で無活性化されます.
研究 の 目的:
- ヒトの冠動脈および頸動脈の損傷における脂質過酸化物含有量に対するPON1同型QとRの効果を比較する.
- 動脈硬化性病変の文脈におけるPON1の酵素的活動を調査する.
主な方法:
- PON1QまたはPON1Rによる冠動脈および頸動脈損傷の同質体のインキュベーション.
- 脂質過酸化物含有量とPON1活性を測定する.
- 水解産物の分析とPON1変異体の影響.
主要な成果:
- PON1QとPON1Rは,冠動脈損傷では最大27%,頸動脈損傷では最大16%の脂質過酸化を減少させた.
- PON1QとPON1Rの不活性化は,それぞれ15%と45%で発生しました.
- PON1は,脂質ヒドロペロキシドを水解および還元することによって,エストラーゼ類およびペロキシダゼ類の活性を示した.
結論:
- PON1は,ヒトの動脈硬化病変内の脂質過酸化物を水解する.
- PON1は,脂質過酸化水解活性により,抗アテロゲン性を持ちます.
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