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Updated: Jun 18, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

RNAポリメラーゼプロモーターの構造的組織は,オープン複合体である

N Naryshkin1, A Revyakin, Y Kim

  • 1Howard Hughes Medical Institute, Department of Chemistry, Rutgers University, Piscataway, New Jersey 08854, USA.

Cell
|July 13, 2000
PubMed
まとめ

研究者らは,細菌のRNAポリメラーゼ (RNAP) とプロモーターDNAの相互作用を,フォトクロスリンクを用いてマッピングした. この詳細な地図は,RNAP-プロモーターオープン複合体 (RPo) のタンパク質-DNA結合と,アクティベータがその構造にどのように影響するかを示しています.

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