ORCA3は,植物初次および二次代謝のジャスモナート反応性転写レギュレータである
1Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands.
Science (New York, N.Y.)
|July 15, 2000
まとめ
植物ストレスホルモンジャスモナートは,二次代謝物質の生物合成を誘発する. ORCA3転写因子の過剰発現は,このプロセスを強化し,Catharanthus roseus.でアルカロイドの蓄積を促進します.
科学分野:
- 植物生物学 植物生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- ジャスモナートは,二次代謝産物を調節する重要な植物ストレスホルモンです.
- 転写因子は,ホルモン反応を媒介する上で重要な役割を果たします.
- カタランタス・ローゼウス (Catharanthus roseus) は,アルカロイド生物合成を研究するためのモデル植物である.
研究 の 目的:
- 二次代謝産物生物合成におけるジャスモナート反応性ORCA3転写因子の役割を調査する.
- ORCA3が代謝遺伝子の発現にどのように影響するかを理解する.
主な方法:
- 移転DNA (T-DNA) アクティベーションタグを使用したORCA3遺伝子の分離.
- カタランタス (Catharanthus roseus) のORCA3遺伝子の過剰発現が確認されました.
- メタボリート生物合成遺伝子発現の分析.
- テルペノイドインドルアルカロイドの蓄積の定量化.
主要な成果:
- ORCA3遺伝子が成功裏に分離されました.
- ORCA3の過剰発現は,複数の代謝生物合成遺伝子の発現を強化しました.
- テルペノイド・インドル・アルカロイドの蓄積は,ORCA3.3を過剰に発現する植物において有意に増加した.
結論:
- ORCA3は,ジャスモナート誘発の二次代謝物質バイオシンセシスの陽性調節剤として作用する.
- ORCA3のようなジャスモネートに反応するAP2ドメインの転写因子は,植物ストレスシグナルを代謝変化と結びつける.
- これは,植物がストレス下での代謝をどのように適応させるかの分子メカニズムを提供します.
関連する概念動画
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
01:31Transducer Mechanism: Nuclear Receptors
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
01:28Global Regulatory Systems
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...