関連する実験動画
Updated: Jul 14, 2026

09:51
Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
Published on: July 16, 2017
CLAVATA3は,CLAVATA1受容体キナーゼのマルチメリックリガンドである
A E Trotochaud1, S Jeong, S E Clark
1Department of Biology, University of Michigan, Ann Arbor, MI 48109-1048, USA.
まとめ
CLAVATA3 (CLV3) タンパク質は,CLAVATA1 (CLV1) 受容体キナーゼと結合し,植物の芽生えの成長を調節する. 細胞のバランスにとって重要なこの相互作用は,CLV3結合のためのCLV1キナーゼ活性を必要とします.
科学分野:
- 植物生物学 植物生物学
- 分子遺伝学 分子遺伝学
- 発達生物学 発達生物学とは
背景:
- CLAVATA1 (CLV1) とCLAVATA3 (CLV3) タンパク質は,アラビドプシスのシートアピカルメリステム (SAM) サイズを制御する重要なレギュレーターです.
- CLV1は受容体のようなキナーゼであり,CLV3は小さな分泌ペプチドであり,リガンド-受容体相互作用を示唆しています.
研究 の 目的:
- CLV1とCLV3タンパク質の相互作用をインビボで調査する.
- CLV1.1に結合するCLV3の機能要件を決定する.
主な方法:
- 同免疫プレシピテーションアッセイは,タンパク質の相互作用を in vivo で検出する.
- 酵母結合測定は,直接的な相互作用を確認し,キナーゼ活性依存性を評価するために行われます.
- 植物抽出物におけるCLV3複合体の形成の分析.
主要な成果:
- CLV3とCLV1のタンパク質は共免疫プレシピテートであり,これは体内の相互作用を示しています.
- CLV1およびCLV2を発現する酵母はCLV3に結合し,この結合はCLV1キナーゼの活性に依存する.
- カリフラワーエキスのCLV3の75%以上はCLV1に結合しており,リガンド結合を示唆しています.
- 溶解性CLV3は,約25 kDaのマルチメリック複合体として存在します.
結論:
- CLV3はCLV1受容体キナーゼのリガンドとして作用する.
- CLV1キナーゼの活性性は,CLV3結合に不可欠であり,下流信号伝達に不可欠である.
- この相互作用は,リガンド連結によるショット・メリステム・ホメオスタシスの維持に重要な役割を果たします.
さらに関連する動画
関連する概念動画
Enzyme-linked Receptors
Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Ligand Binding and Linkage
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence the...
ATP Driven Pumps III: V-type Pumps
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Receptor Tyrosine Kinases
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

