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表現プロファイルの要約による機能的発見
T R Hughes1, M J Marton, A R Jones
1Rosetta Inpharmatics, Inc., Kirkland, Washington 98034, USA.
Cell
|August 10, 2000
まとめ
この研究は,何百もの細胞機能を同時に監視するための新しい測定法を導入しています. この方法は,遺伝子発現プロフィールを分析することによって,遺伝子機能と薬物効果を理解するのに役立ちます.
科学分野:
- システム生物学 システム生物学
- 分子生物学は分子生物学である.
- ゲノミクスゲノミクスとは
背景:
- 混乱に対する細胞の反応を理解することは,生物学において極めて重要です.
- 既存の方法は,多数の細胞機能を同時に監視する能力が欠けていることが多い.
研究 の 目的:
- 何百もの細胞機能を同時にモニタリングするための単一のアッセイを開発する.
- さまざまな波動に対する表現プロファイルの参照集を作成する.
- 特徴づけられていない遺伝子と薬理学的剤の特徴づけを可能にする.
主な方法:
- *Saccharomyces cerevisiae* (酵母) の300の変異と化学処理に関する遺伝子発現プロフィールのリファレンスコンペンドムの構築.
- 表現プロファイルのパターンマッチング分析を使用して,影響を受けた細胞経路を特定します.
- 特定された遺伝子機能の実験的検証.
主要な成果:
- パターンマッチングを通じて,微妙なものであっても,混乱の影響を受ける細胞経路を成功裏に特定しました.
- 特徴づけられていない8つの遺伝子を特徴付け,ステロール代謝,細胞壁機能,ミトコンドリア呼吸,またはタンパク質合成における役割を割り当てました.
- 薬物ダイクロニンの新しい標的を特定し,薬理学研究におけるコンペンドムの有用性を実証した.
結論:
- 表現プロファイルの要約と組み合わせた単一のアッセイは,細胞機能を解剖するための強力なツールを提供します.
- このアプローチは,特徴づけられていない遺伝子を効果的に特徴付け,薬物作用のメカニズムを理解するのに役立ちます.
- 開発された方法は,細胞の干渉のシステムレベルの分析のためのスケーラブルなソリューションを提供します.
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