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Wnt信号伝達によるアディポゲネシスの抑制
S E Ross1, N Hemati, K A Longo
1Department of Physiology, Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109-0622, USA.
Science (New York, N.Y.)
|August 11, 2000
まとめ
Wntシグナリングは,脂肪細胞形成 (アディポゲネシス) の分子スイッチとして作用します. Wntシグナル伝達を阻害すると,先駆体細胞の分化がアディポサイトに促進され,細胞の運命決定に影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
- 細胞生物学 細胞生物学
背景:
- Wntシグナル伝達タンパク質は,様々な発達過程の重要な調節因子である.
- 脂肪細胞の分化過程であるアディポゲネシスは,特定の転写因子を含む複雑な経路です.
研究 の 目的:
- アディポゲネシスを支配する分子スイッチとしてのWnt信号伝達の役割を調査する.
- Wntシグナル伝達がプレアディポサイトや他のメソダーマ細胞の分化にどのように影響するかを決定する.
主な方法:
- Wntシグナル伝達阻害体であるアクシンが過剰発現する.
- 支配的負のTCF4の表現は,Wnt信号をブロックする.
- プレアディポサイトとミオブラストの微分化の分析 in vitro.
主要な成果:
- Wntシグナリングは,主要なアディポゲン転写因子,CCAAT/エンハンサー結合タンパク質アルファ (C/EBPalpha) およびペロキシソーム増殖器活性化受容体ガンマ (PPARgamma) を阻害することによって,プレアディポサイトを無差別状態に保ちます.
- アクシン過剰発現またはドミナントネガティブなTCF4によって誘発されたWntシグナル伝達を防止する. プレアディポサイトにおけるアディポサイト分化.
- Wntシグナル伝達の障害は,ミオブラストをアディポサイトに変異させた.
結論:
- Wnt信号は,アディポゲネシスを制御する重要な分子スイッチです.
- Wntのシグナル伝達阻害は,プレアディポサイトからアディポサイトの分化を誘導するのに十分である.
- Wnt経路は,中皮細胞の運命を決定する上で重要な役割を果たし,アディポサイトを含む様々な細胞タイプの分化に影響を与えます.
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