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Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Chromosome Structure02:40

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Replication02:31

Chromosome Replication

Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin of...
Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

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Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
17:14

Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

Published on: December 10, 2012

イーストの染色体複製における時間的な順序

W Burke, W L Fangman

    Cell
    |July 1, 1975
    PubMed
    まとめ
    この要約は機械生成です。

    N-メチル-N'-ニトロ-N-ニトロスグアニジン (NG) 変異は,酵母における一時的なゲノム複製を明らかにする. 特定の遺伝子は,DNA合成の明確な間隔の間にNGに対するピーク感受性を示し,酵母ゲノム全体で秩序ある複製を示す.

    さらに関連する動画

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    関連する実験動画

    Last Updated: Jun 16, 2026

    Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization
    17:14

    Chromosome Replicating Timing Combined with Fluorescent In situ Hybridization

    Published on: December 10, 2012

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
    08:40

    Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

    Published on: October 21, 2022

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
    07:48

    Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

    Published on: October 11, 2022

    科学分野:

    • 分子生物学は分子生物学である.
    • 遺伝学 遺伝学とは
    • 微生物学 微生物学とは

    背景:

    • N-メチル-N'-ニトロ-N-ニトロスグアニジン (NG) への遺伝子の感受性は,細菌の複製タイミングと相関している.
    • ゲノム複製順序を理解することは,真核細胞生物学にとって極めて重要です.

    研究 の 目的:

    • 単細胞ユーカリオット,Saccharomyces cerevisiae (酵母) のゲノム複製の時間的な順序を調査する.
    • 特定の遺伝子がDNA合成期間中に,事前に決定された時間に複製するかどうかを決定する.

    主な方法:

    • N-メチル-N'-ニトロ-N-ニトロスグアニジン (NG) を遺伝子感受性を評価するための変異因子として利用しました.
    • 指数関数的に成長する酵母細胞と,DNA合成が阻害された細胞の変異遺伝感を比較した.
    • 酵母細胞集団を同期して分析し,NGに対する最大遺伝マーカーの感受性の明確な間隔を特定しました.

    主要な成果:

    • 指数関数的に成長する酵母細胞は,抑制されたDNA合成を持つ細胞よりもNG変異に敏感でした.
    • 同期した細胞の個々の遺伝子マーカーは,DNA合成期間の間の離散的な間隔で最大NG感受性を示した.
    • 4つの染色体における5つの遺伝子のマーカーは,異なる感受性ピークを示し,一時的な複製順序を示唆した.

    結論:

    • この発見は,酵母遺伝子のほとんどの遺伝子の複製の時間的な順序を示しています.
    • DNA複製のための特定のイニシアチブサイトは,酵母染色体DNAの定義された領域に存在する可能性が高い.
    • これらの複製開始部位は,DNA合成期間中に,事前に決定された時点で活性化されます.