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Updated: May 11, 2026

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
08:13

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Published on: September 1, 2018

エシェリキア・コライ菌の変異体は,一部の転送RNA前駆分子から3'端核酸を除去する機能に欠陥がある

J G Seidman, F J Schmidt, K Foss

    Cell
    |August 1, 1975
    PubMed
    まとめ

    バクテリオファグのT4RNA処理には,特定の酵素が必要です. 変異したEscherichia coli菌株がこれを阻害し,少なくとも2つの3'-リボ核酸が転送RNAの成熟に不可欠であることを示しています.

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    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli07:04

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

    Published on: February 5, 2019

    Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility12:29

    Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

    Published on: March 11, 2022

    関連する概念動画

    Mismatch Repair01:36

    Mismatch Repair

    Overview
    Nuclear Export of mRNA02:31

    Nuclear Export of mRNA

    Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
    Transfer RNA Synthesis02:36

    Transfer RNA Synthesis

    One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
    Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
    Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
    Nonsense-mediated mRNA Decay02:27

    Nonsense-mediated mRNA Decay

    The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
    Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
    Mismatch Repair01:20

    Mismatch Repair

    Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
    The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
    The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...