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Updated: Jul 8, 2026

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Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
Published on: July 6, 2022
成体体体細胞から核移転によって生成されたクローン豚
I A Polejaeva1, S H Chen, T D Vaught
1PPL Therapeutics Incorporated, Blacksburg, Virginia 24060, USA. ipolejaeva@ppl-therapeutics.com
Nature
|September 19, 2000
まとめ
研究者は,新しい核移転技術を使用して成体体細胞からブタをクローンに成功し,ブタのクローンにおける以前の課題を克服しました. この画期的な発見は,豚の体細胞核移転 (SCNT) を進めている.
科学分野:
- 生殖生物学 生殖生物学
- 発達生物学 発達生物学とは
- 動物のクローニング
背景:
- ソマティック細胞核移転 (SCNT) は,1995年以来,様々な哺乳類の種で生きた子孫を成功裏に生み出してきました.
- 豚のSCNTは,他の種と比較して著しく成功していないが,体細胞を用いての成功は限られている.
研究 の 目的:
- 核移植における胚の発達に影響を与える要因を調査する.
- 新しいSCNT手順を使用して,培養成体体細胞からクローンされたブタの子豚の成功生産を報告する.
主な方法:
- 核移植は,二倍体ドナー核を核のないMII卵細胞に移植することを含む.
- 再構築された胚の活性化と,その後の代理受容者に移植.
- 豚のクローニングのための新しい核移転手法の開発と適用.
主要な成果:
- 培養成体体細胞集団からクローンされたブタの子豚の成功生産.
- ソマティック細胞を用いた豚のSCNTにおける以前の制限を克服しました.
- 新しい核移転手順の有効性を実証しました.
結論:
- 開発された核移転手順は,成人体細胞からクローンされたブタを生産するのに有効です.
- この研究は,豚のクローン技術における重要な進歩を表しています.
- 豚のSCNT効率に影響を与える要因に関するさらなる調査が必要である.
関連する概念動画
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Introduction to Nuclear Reprogramming
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
Cloning of Dolly the Sheep
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter telomeres than other...
Somatic to iPS Cell Reprogramming
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

