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関連する概念動画

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening06:00

Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening

858
This protocol describes the assembly of a pneumatic system for the delivery of pressurized air to a needle during the process of needle beveling. The protocol further describes the beveling process for creating sharp microinjection needles and how to gauge the relative opening size of the...
858
Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels08:12

Fully Endoscopic Mitral Valve Repair with Percutaneous Cannulation of Groin Vessels

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This protocol demonstrates in detail how to perform fully endoscopic mitral valve surgery (EMS) with percutaneous cannulation of the groin vessels, using a percutaneous plug-based vascular closure device. Fundamental steps and useful instructions are described in detail for each step.
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A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy03:56

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy

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This "two-step method" significantly improved the success rate of percutaneous transhepatic choledochoscopy and achieved a better prognosis of intrahepatic and extrahepatic...
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Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)05:39

Three-Dimensional Printing Guide Template Assisted Percutaneous Vertebroplasty (PVP)

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Herein, we present a three-dimensional printing guide template for percutaneous vertebraplasty. A patient with a T11 vertebral compression fracture was selected as a case...
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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

9.3K
Optical coherence tomography (OCT) is a high resolution imaging technique that allows analysis of tissue specific optical properties providing the means for tissue differentiation. We developed needle based OCT, providing real-time imaging combined with on-the-spot tumor differentiation. This publication describes a method for percutaneous, needle based OCT of renal...
9.3K
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy11:27

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

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Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research...
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関連する実験動画

Updated: Jan 30, 2026

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy
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ピルスの収縮は,細菌の動性や動きに力を与えます.

A J Merz1, M So, M P Sheetz

  • 1Department of Molecular Microbiology and Immunology, Oregon Health Sciences University, Portland 97201-3098, USA.

Nature
|September 19, 2000
PubMed
まとめ

タイプIV pili (Tfp) は力強く収縮し,細菌細胞の移動と表面の爬行を直接媒介する重要な力を発生します. この収縮メカニズムは,バクテリアの運動性と微小コロニーの形成に不可欠です.

科学分野:

  • 微生物学 微生物学とは
  • 細胞生物学 細胞生物学
  • バイオフィジックス 生物物理学

背景:

  • グラム陰性バクテリアは,表面転移のために,震えと社会的滑り運動性を利用します.
  • タイプIV pili (Tfp) は,これらの運動性メカニズムに不可欠ですが,その正確な役割は不明です.

研究 の 目的:

  • IV型ピリ (Tfp) が細菌の運動を媒介するメカニズムを解明する.
  • 細菌の表面移動中にTfpによって生成される力を定量化するために.

主な方法:

  • レーザーピンチを使って,個々の細菌細胞とそのTfpが及ぼす力を操作し,測定しました.
  • Tfp-ビーズの相互作用に関する定量的な力測定を行った.
  • ガラスの表面でのバクテリアの爬行と微小コロニー形成が観察されました.

主要な成果:

  • Tfpが強引に収縮し,細菌細胞を微小コロニーに積極的に引き寄せることを示した.
  • Tfpがラテックスビーズに結合すると,80pNを超える回収力が測定されています.
  • Tfp媒介の運動性と収縮は,約1ミクロン/秒で発生し,タンパク質合成とPilTタンパク質に依存していることが確認されました.

さらに関連する動画

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

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関連する実験動画

Last Updated: Jan 30, 2026

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy
03:56

A Two-Step Method for Percutaneous Transhepatic Choledochoscopic Lithotomy

Published on: September 13, 2022

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In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses
09:31

In Vivo, Percutaneous, Needle Based, Optical Coherence Tomography of Renal Masses

Published on: March 30, 2015

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Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
11:27

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Published on: February 7, 2015

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結論:

  • タイプIV pili (Tfp) が積極的に収縮し,かなりの力を生み出すことが判明しました.
  • Tfp収縮が細菌の運動性と表面の爬行を促す直接的なメカニズムであると結論付けました.
  • Tfp媒介による力発生と運動における PilT タンパク質機能の重要性を強調した.