ニューロンにおけるグルタマタージック現象型を定義する膀性グルタマートトランスポーターの識別
S Takamori1, J S Rhee, C Rosenmund
1Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, Göttingen, Germany.
Nature
|September 23, 2000
まとめ
脳の細胞は,ブドウ状グルタミン酸トランスポーターを使用して,グルタミン酸を包装し,主要な興奮神経伝達物質である. 研究者らは,BNPIを発現させることで,細胞内小胞がグルタミン酸を輸送し,グルタマタージック現象型を定義することを発見した.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- グルタミン酸は,哺乳類の中央神経系における主要な興奮神経伝達物質である.
- 膀搬送器は,神経伝達物質をシナプス膀に負荷するために不可欠です.
研究 の 目的:
- グルタミン酸の吸収におけるBNPI (膀結合トランスポーター) の機能を調査する.
- BNPI発現がグルタマタージック現象型を与えるのに十分かどうかを判断する.
主な方法:
- 細胞とニューロンで発現したBNPI.
- 刺激されたエクソサイトーシスと測定されたグルタミン酸の放出.
- グルタミン酸およびGABA受容体アンタゴニストを使用して,ポストシナプス電流を分析しました.
主要な成果:
- BNPI発現は,シナプスベジクルに類似した特性を持つ細胞内小胞にグルタミン酸の吸収をもたらした.
- BNPIを発現する細胞を刺激すると,グルタミン酸の量的な放出が生じる.
- グルタミン酸はGABAではなく,BNPI発現ニューロンのポストシナプス電流の成分を媒介した.
結論:
- BNPIはベジキュラーグルタミン酸トランスポーターとして機能します.
- BNPIの発現は,ニューロンにおけるグルタマタージック現象型を確立するのに十分である.
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Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...


