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LDL受容体に関連するタンパク質が,マウスのWntシグナル伝達を媒介する
K I Pinson1, J Brennan, S Monkley
1Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Nature
|October 12, 2000
まとめ
低密度リポプロテイン受容体関連タンパク質6 (LRP6) は,哺乳類のWnt信号伝達に不可欠である. その変異は,発達障害を引き起こし,LRP6を強調します.
科学分野:
- 発達生物学 発達生物学について
- 分子遺伝学 分子遺伝学
- 細胞シグナル伝達 細胞信号伝達
背景:
- Wntタンパク質は,胚の発達における重要な分泌因子であり,体軸のパターンを調節し,中枢神経系と四肢の発達,および器官生成を制御する.
- Wntシグナル伝達経路は部分的に理解されているが,Wntタンパク質とそのFrizzled受容体が信号を下流エフェクタに送信するメカニズムは不明である.
研究 の 目的:
- 哺乳類の発達中のWnt信号伝達における,低密度リポプロテイン (LDL) 受容体関連タンパク質ファミリーの新しいメンバー,LRP6の役割を調査する.
主な方法:
- LRP6遺伝子の挿入変異を有するマウス胚の分析.
- LRP6欠陥の胚における発達障害の検査.
- 異なるLRP6遺伝子コピー数を持つマウスにおけるWnt変異フェノタイプの遺伝子解析.
主要な成果:
- ホモジゴス型LRP6欠乏マウス胚は,個々のWnt遺伝子の変異で見られる欠陥を反映する発達の異常を示します.
- 減少したLRP6遺伝子用量は,既存のWnt変異のフェノタイプを遺伝的に強化する.
結論:
- LRP6は,哺乳類における複数のWntリガンドからの信号を伝達する上で重要な役割を果たします.
- LRP6は正常な胚の発達に不可欠であり,Wnt信号伝導の重要な構成要素として機能します.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...