Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Death and the cell.

Immunology today·2014
Same author

Killer immunoglobulin-like receptor gene repertoire influences viral load of primary human cytomegalovirus infection in renal transplant patients.

Genes and immunity·2014
Same author

p62/Sequestosome-1: Mapping Sites of Protein-Handling Stress in Canine Cutaneous Mast Cell Tumors.

Veterinary pathology·2014
Same author

In utero gene therapy rescues microcephaly caused by Pqbp1-hypofunction in neural stem progenitor cells.

Molecular psychiatry·2014
Same author

Science-in-brief: What is needed to prevent tendon injury in equine athletes? A conversation between researchers and industry stakeholders.

Equine veterinary journal·2014
Same author

An analysis of the relative activities of a number of promoter constructs from genes which are expressed during late pollen development as determined by particle bombardment.

Plant cell reports·2013
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
Same journal

China boosts prestigious grants for young scientists - will it ease competition?

Nature·2026
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
関連記事をすべて見る

関連する実験動画

Updated: May 10, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

DNAが損傷した後に死と闘う.

T Rich1, R L Allen, A H Wyllie

  • 1Department of Pathology, University of Cambridge, UK.

Nature
|October 26, 2000
PubMed
まとめ
この要約は機械生成です。

DNAの損傷はしばしばプログラム細胞死 (アポトーシス) を引き起こす. この研究では,DNAの損傷がアポトーシスを開始する理由,細胞死亡の代替方法,および関連するシグナル伝達経路について調べています.

さらに関連する動画

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

関連する実験動画

Last Updated: May 10, 2026

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • DNAの損傷は,細胞の重要な侮辱である.
  • アポトーシス (プログラム細胞死) は,DNA損傷に対する一般的な反応です.
  • アポプトーシスを受けるという決定は複雑で,様々な細胞信号を統合することを含む.

研究 の 目的:

  • DNA損傷がアポトーシスを引き起こす根本的な理由を調査する.
  • DNAに損傷した細胞の代替運命を調査し,これらの選択に影響を与える要因を探求する.
  • DNAの損傷を検知し,アポプトシス経路に影響を与えるシグナル伝達メカニズムを解明する.
  • 核アポプトソーム複合体の潜在的な存在を調査する.
  • DNA損傷後のアポトーシスの失敗の結果を理解するために.

主な方法:

  • この研究は主に理論的であり,概念的分析と既存の知識の統合を通じて根本的な問題に取り組んでいます.
  • DNA損傷反応とアポトーシスに関連するシグナル伝達経路と分子メカニズムを調査する.
  • この研究は,細胞の運命決定に影響を与える細胞内および細胞外刺激に関する情報を合成します.

主要な成果:

  • DNA損傷は,潜在的に有害な突然変異に対する保護機構としてアポトーシスを開始します.
  • 細胞は,損傷の重度とシグナル伝達に基づいて,アポトーシスとDNA修復や細胞サイクル停止などの他の運命を選択することができます.
  • 特定の分子信号がDNAの損傷を認識し,下流のアポプトシスエフェクターを活性化します.
  • 核アポトソーム複合体の存在と機能は,さらなる調査のための分野であり続ける.
  • アポプトーシスを開始できない場合,ゲノム不安定と癌などの病気を引き起こす可能性があります.

結論:

  • アポトーシスはDNA損傷に対する重要な保護策ですが,代替的な細胞反応も不可欠です.
  • DNA損傷後の細胞運命を決定する複雑な信号ネットワークを理解することは,細胞の健康と病気を理解するために不可欠です.
  • 核アポプトシス機構とDNA損傷反応におけるその役割の完全な特徴を明らかにするために,さらなる研究が必要である.