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Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
翻訳的に結合されたmRNAのターンオーバーのためのメカニズム:ポリ (A) 尾と,タンパク質複合体の経由でc-fos RNAのコーディング決定体との相互作用
1Department of Biochemistry and Molecular Biology, The University of Texas Houston Medical School 77030, USA.
Cell
|October 29, 2000
まとめ
c-fos mRNAの主要なタンパク質コーディング領域の不安定性決定因子 (mCRD) は,その距離からポリー (A) 尾まで依存しています. Unr,PABP,PAIP-1,hnRNP D,NSAP1を含むタンパク質複合体は,デデニレーションを阻害することによってmRNAを安定させます.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- RNAの代謝について
背景:
- mRNAのターンオーバーは,遺伝子発現の調節に不可欠です.
- c-fos プロトオンコゲントランスクリプトの主要なタンパク質コーディング領域の不安定性決定因子 (mCRD) は,mRNAの安定性に影響する.
- mRNAの分解のメカニズムを理解することは,細胞のプロセスを理解するために不可欠です.
研究 の 目的:
- mRNAのターンオーバーにおけるmCRDの役割を調査する.
- mCRDと関連するタンパク質とその機能を特定するために.
- mRNAのターンオーバーとトランスレーションの相互作用を解明する.
主な方法:
- 生化学技術を用いたmCRD関連タンパク質の識別.
- タンパク質複合体調節への反応としてmRNAの安定性の分析.
- ポリ (A) 尾からmCRDの距離が機能に与える影響を調査する.
主要な成果:
- mCRDの機能は,poly (A) tail.に近いところに依存しています.
- 5つのタンパク質 (Unr, PABP, PAIP-1, hnRNP D, NSAP1) はmCRDに関連したタンパク質として識別され,マルチタンパク質複合体を形成しました.
- これらのタンパク質の過剰発現は,デデエニレーションを阻害することによって,mCRDを含むmRNAを安定させました.
結論:
- ブリッジング・コンプレックスが,ポリー (A) テイルとmCRD.の間で形成されます.
- リボソームのトランジットは,この複合体を破壊または再編成し,mRNAのデデデニレーションと衰退につながります.
- この研究は,mCRDによって媒介される翻訳依存的衰退を含むmRNA調節の新しいメカニズムを明らかにしています.
関連する概念動画
The Central Dogma
Overview
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
The Central Dogma
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
Regulated mRNA Transport
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

