マトリックスタンパク質は,ゴルギ装置の高階構造を生成することができる
J Seemann1, E Jokitalo, M Pypaert
1Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8002, USA.
Nature
|November 9, 2000
まとめ
細胞の臓器であるゴルギ装置は,酵素から独立してその構造を維持することができる. これは,ゴルギが自律的な支架であり,エンドプラズマ網膜のダイナミックな部分だけではないことを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子および細胞生物学
背景:
- ゴルジ装置は動物細胞の重要な器官で,堆積した水槽のネットワークを形成します.
- その構造は,細胞骨格とマトリックスタンパク質,例えばゴルギンとGRASPタンパク質に依存すると考えられている.
研究 の 目的:
- ゴルギ装置の酵素成分からの構造的独立性を調査する.
- ゴルギ建築の維持におけるマトリックスタンパク質の役割を調査する.
主な方法:
- オリゴサッカリド改変酵素からゴルジ基質タンパク質を分離する.
- ゴルギ網膜の分離後の構造的整合性と局所化の評価.
主要な成果:
- ゴルギマトリックスタンパク質 (ゴルギンとGRASPタンパク質) は,ゴルギ居住酵素から成功裏に分離されました.
- ゴルギ装置の構造的支架は,酵素を除去した後も,リボン状の網膜とユクスタヌクレアロケーションを維持した.
- 分離されたマトリックスタンパク質は,ゴルギの構造を形成する能力を保持しました.
結論:
- ゴルジ装置は,その酵素含有量とは独立して存在できる固有の構造的支架を持っています.
- これは,ゴルギがエンドプラズマ網膜と単純な動的均衡状態にあるのではなく,自律的な器官であることを示唆しています.
- ゴルギは,貨物の改変に関与する酵素を含む膜で満たされた構造的枠組みとして機能する可能性があります.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
01:11Formation of Higher-order Actin Filaments
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The high-order actin networks...
The high-order actin networks...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...