Isw2染色体改造複合体は,Ume6pによって採用されると,初期のメオティック遺伝子を抑制する

J P Goldmark¹, T G Fazzio, P W Estep

¹Division of Basic Sciences, Fred Hutchinson Cancer Research Institute, Fred Hutchinson Cancer Research Center and University of Washington, Seattle 98109, USA.

Cell

|November 18, 2000

まとめ

イーストのIsw2複合体 (ISWI) は,細胞分裂中の早期の中性遺伝子転写を抑制する. この染色体改造はUme6pに依存し,アクセス不可能なDNA構造を確立します.

科学分野:

分子生物学は分子生物学である.
クロマチンの生物学
イースト遺伝学イースト遺伝学

背景:

ISWI染色体リモデレータは in vitro 活性を示しているが,in vivo の機能は不明である.
in vivoの役割を理解することは,遺伝子調節を理解するために不可欠です.

研究の目的:

クロマチンの改造因子のISWIクラスのin vivo機能を解明する.
S. cerevisiae Isw2複合体による転写抑制の分子機構を調査する.

主な方法:

S. cerevisiae.の遺伝子発現の変化を調査した.
クロマチンの構造を in vivo で分析するために使用された核酵素消化アッセイ.
Isw2複合体の募集におけるUme6pの役割を調べました.

主要な成果:

S. cerevisiae Isw2複合体は,ミトスの成長中の早期の中間遺伝子転写を抑制する.
この抑制は,Rpd3-Sin3ヒストン脱酸化酵素複合体への平行経路で発生する.
Isw2複合体媒介抑制はUme6pに依存しており,Ume6pは複合体を標的遺伝子に勧誘する.
核酵素の消化により,Isw2複合体は,Ume6p結合部位の近くで核酵素がアクセスできないクロマチンを形成することが明らかになった.

結論:

Isw2複合体は,体内での転写抑制剤として機能する.
異なった染色体改造複合体を含む転写抑制のモデルが提案されています.
Ume6pは,Isw2複合体を特定の遺伝子にターゲティングするための重要なレギュラーです.

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