PCNAはDNA複製を酵母における表遺伝子遺伝と結びつける
Z Zhang1, K Shibahara, B Stillman
1Cold Spring Harbor Laboratory, New York 11724, USA.
Nature
|November 23, 2000
まとめ
増殖細胞核抗原 (PCNA) の突然変異は,酵母におけるテロメアと交配型ロシ近くでの表遺伝子の遺伝子静止を妨害する. PCNAは,細胞分裂中のDNAとクロマチンの構造の両方を継承するのに不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
- イースト遺伝学 イースト遺伝学
背景:
- Saccharomyces cerevisiaeの転写サイレンシングは,HM交配型ロシやテロメアのヘテロクロマチンのような構造によって媒介されます.
- これらのエピジェネティック構造は,複数のミトス分裂を通して安定的に継承されます.
研究 の 目的:
- 増殖細胞核抗原 (PCNA) が表遺伝子遺伝子の静止化を維持する役割を調査する.
- PCNAが染色体構造の遺伝に影響を与えるメカニズムを解明する.
主な方法:
- テロメアとHMRロカスにおける遺伝子サイレンシングの欠陥に対するPCNA変異体 (pol30-8,pol30-6,pol30-79) の分析.
- ADE2遺伝子レポーターシステムを用いて静音化の測定.
- クロマチンアセンブリファクター1 (CAF-1) とのインビトロ結合測定とインビボクロマチンの関連研究.
主要な成果:
- PCNA変異は,テロメア付近とHMRロカスで遺伝子の抑制を低下させた.
- pol30-8変異体は安定した静止力の喪失を示し,その結果,抑制された細胞と抑制された細胞の混合が生じた.
- 他のPCNA変異種は,CAF-1の枯渇,CAF-1への結合の減少,およびCAF-1クロマチンの関連性の変化とともに,相乗効果の欠陥を示した.
結論:
- PCNAは,S段階におけるエピジェネティッククロマチン構造の継承において重要な役割を果たします.
- PCNAは,CAF-1を含む少なくとも2つの異なるメカニズムを通じて,遺伝子サイレンスに寄与する.
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