関連する実験動画
Updated: Jul 13, 2026

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Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
DNA結合タンパク質のゲノム全体の位置と機能
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
まとめ
研究者は,DNA結合タンパク質を in vivo でマッピングするための新しいマイクロアレイ方法を開発しました. この技術は,特定のアクティベーターによって制御される遺伝子を特定し,酵母における新しい機能と規制ネットワークを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- システム生物学 システム生物学
背景:
- DNA結合タンパク質の機能を理解することは,遺伝子発現とゲノム維持の解読に不可欠です.
- これらのタンパク質の in vivo 染色体位置を特定することは,この理解のために不可欠です.
研究 の 目的:
- ゲノム全体にわたるDNA結合タンパク質の局所化のための新しいマイクロアレイ法を開発し,適用する.
- 酵母におけるトランスクリプション活性化剤Gal4とSte12の直接標的と規制作用を調査する.
主な方法:
- ゲノム全体のDNA結合タンパク質の位置分析のためのマイクロアレイベースの方法の開発.
- 位置データを遺伝子発現プロファイルと統合する.
- 酵母における転写活性化剤Gal4とSte12の結合のモニタリング.
主要な成果:
- Gal4 と Ste12 のゲノム全体の結合部位の特定.
- タンパク質結合と遺伝子発現の相関は,環境のシグナル (炭素源,交配フェロモン) に反応して変化する.
- Gal4とSte12の協調調節経路と以前未知の機能の発見
結論:
- ゲノム全体の位置分析は,遺伝子規制ネットワークを研究するための強力なツールです.
- このアプローチは,遺伝子機能とゲノム維持機構の調査を容易にする.
- この研究は,細胞反応における特定の転写活性化剤の役割に関する新しい洞察を明らかにしています.
関連する概念動画
Organization of Genes
Overview
Nucleic Acids
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Organization of Genes
Overview
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Structure of a Gene
A gene is the fundamental unit of heredity. Every individual has two copies of each gene, one inherited from each parent. Although most people contain the same genes, there is a small fraction that is slightly different amongst people. A gene with a small difference in its sequence of DNA bases forms different alleles, contributing to different phenotypes.
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...

