Mop3は,哺乳類のマスターサーカディアンペースメーカーの重要な構成要素です
M K Bunger1, L D Wilsbacher, S M Moran
1The McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison, WI 53706, USA.
Cell
|February 13, 2001
まとめ
MOP3 (BMAL1とも呼ばれる) タンパク質が欠けているマウスは,日中リズムを完全に失い,活動に障害を示します. これは,MOP3が哺乳類の生物学的時計に不可欠であることを示しています.
科学分野:
- クロノバイオロジーはクロノバイオロジーを用います.
- 分子生物学は分子生物学である.
- 哺乳類生理学 哺乳類生理学
背景:
- シルカディアンリズムは哺乳類の生理学と行動に根本的な役割を果たし,内生的な生物学的時計によって動かされます.
- 昼夜リズムが根底にある分子機構には,複雑なタンパク質の相互作用と遺伝子発現パターンが含まれる.
研究 の 目的:
- 哺乳類における昼夜リズムを調節するPASタンパク質MOP3 (BMAL1とも呼ばれる) の役割を調査する.
- 哺乳類の昼間ペースメーカーの機能にMOP3が不可欠であるかどうかを判断する.
主な方法:
- Mop3 ノックアウト (Mop3-/-) マウスの世代.
- 絶え間ない暗闇における昼夜リズムと,光暗サイクル下での運動運動の評価.
- 上核 (SCN) の周期性遺伝子発現の分析.
主要な成果:
- Mop3-/-マウスは,絶え間ない暗闇の中で昼夜リズムを完全に失っていた.
- ロコモーター活動が低下し,光と暗のサイクル下でMop3-/-マウスの全体的な活動レベルが低下しました.
- SCNにおける期間遺伝子発現分析は,分子昼間機能の喪失を確認した.
結論:
- MOP3 (BMAL1) は,mCLOCKの必須ヘテロジメアパートナーとして遺伝的に検証されています.
- MOP3は,哺乳類の昼間ペースメーカーの非冗長かつ重要な構成要素です.
- MOP3機能の喪失は,昼間の行動と分子時計のメカニズムに深刻な障害をもたらします.
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