関連する実験動画
Updated: Jun 24, 2026
13:51
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
レトロウイルスの遺伝子移植によって成熟した卵細胞に産生されたトランス遺伝子類のサル
A W Chan1, K Y Chong, C Martinovich
1Oregon Regional Primate Research Center, Center for Women's Health, and Departments of Cell-Developmental Biology and Obstetrics-Gynecology, Oregon Health Sciences University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
Science (New York, N.Y.)
|February 24, 2001
まとめ
研究者らは,レトロウイルスベクターと細胞内プラズマ性精子注射を使用して,トランスジェニックのレサス猿を作成しました. 1匹のオスのサルと2匹のツインセットは,緑色光タンパク質 (GFP) の遺伝子統合と発現に成功しました.
科学分野:
- 生殖生物学 生殖生物学
- 遺伝学 遺伝学とは
- 靈長類の研究は,霊長類の研究である.
背景:
- トランスジェニックの動物モデルは,遺伝子機能と病気の研究に不可欠です.
- 生物医学の研究には,トランスジェニックな非ヒト霊長類を生成するための効率的な方法が必要です.
研究 の 目的:
- レトロウイルスベクトルを使用して,トランスジェニックのレサス猿を生成する方法を開発する.
- 緑色光タンパク質 (GFP) トランスゲンの統合と発現を,結果として生まれる子孫で確認する.
主な方法:
- 擬似複製-欠陥のあるレトロウイルスベクターの注射を rhesus卵細胞に.
- 受精のための細胞内プラズマ性精子注射.
- トランスゲンDNA検出のためのサザンブレット分析.
- トランスゲンメッセンジャーRNAと発現のための光成像の分析.
主要な成果:
- 20個の胚移植で生まれた3人の男性の子孫のうちの1人は,トランスジェニックであることが確認されました.
- 73日目に流産した双子の兄弟組は,分析されたすべての組織にトランスゲンの存在を示しました.
- 緑色光タンパク質 (GFP) のレポーター発現は,光画像を用いて検出されました.
結論:
- この研究は,レトロウイルスベクトルアプローチを使用して,トランスジェニックのレサス猿の成功した生成を実証しています.
- この発見は,研究のために遺伝子組み換え霊長類を創出するためのこの方法の可能性を支持しています.
- トランスゲンの統合と発現の成功は,生成された動物モデルで確認されました.
関連する概念動画
01:10Retrovirus Life Cycles
Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the retrovirus to...
01:33Bacterial Transformation
In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
02:33Retroviruses
Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
03:08LTR Retrotransposons
LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
01:16Transduction
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...