関連する実験動画
Updated: Jun 21, 2026
11:23
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Published on: May 22, 2012
G1からS相への細胞サイクル進行のためのセンターソーマル活動の必要性
E H Hinchcliffe1, F J Miller, M Cham
1Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA., Laboratory of Cell Regulation, Wadsworth Center, Albany, NY 12201, USA.
Science (New York, N.Y.)
|February 27, 2001
まとめ
セントロソームは,G1からS段階への細胞サイクル進行に不可欠です. セントロソームを除去すると,細胞がミトーシス後S相に入ることを防ぎ,細胞分裂の調節における重要な役割を強調します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- セントロソームは,動物細胞の主要な微小管組織センターとして機能する.
- 細胞周期調節における,特に体細胞における,その役割は極めて重要であるが,完全に理解されていない.
研究 の 目的:
- 細胞サイクル進行におけるセンターソームの特定の機能を調査する.
- 細胞サイクルのすべての段階や特定の移行にセンターソームが必要かどうかを判断する.
主な方法:
- Sフェーズが完了する前に,BSC-1のアフリカ緑猿の腎臓細胞からセンターソームのマイクロ外科的除去.
- コントロール細胞と比較して,アセントロソーマ細胞 (カリオプラスト) の細胞サイクル進行の観察.
主要な成果:
- アセントロソーマ細胞はミトーシスを完了したが,次のS相に入る前に停止した.
- 転移後のカリオプラストは微小管組織センターを形成したが,セントリオルを再生することはできなかった.
- センターソームの除去なしの対照細胞は,正常に分裂した.
結論:
- 微小管組織センターとは異なるコア・センターソーマル構造は,G1からS段階への移行に必要である.
- これらのコア構造は,細胞がS相に入ると,G2からM相への移行に不可欠ではありません.
関連する概念動画
00:54Interphase
The cell cycle occurs over approximately 24 hours (in a typical human cell) and in two distinct stages: interphase, which includes three phases of the cell cycle (G1, S, and G2), and mitosis (M). During interphase, which takes up about 95 percent of the duration of the eukaryotic cell cycle, cells grow and replicate their DNA in preparation for mitosis.
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
02:36Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
02:11The Cell Cycle Control System
The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
02:36Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
01:28The Cell Cycle Control System
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...