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関連する概念動画

DNA Microarrays02:34

DNA Microarrays

20.6K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.6K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

20.5K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
20.5K
Genomics02:02

Genomics

39.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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An improved synthesis of the saponin, polyphyllin D.

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Noninvasive diagnosis of renal-allograft rejection by measurement of messenger RNA for perforin and granzyme B in urine.

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MHC-independent allograft vascular disease: mRNA profile in the MHC congenic rat heterotopic cardiac transplant model.

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関連する実験動画

Updated: Jan 8, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

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マイクロアレイ技術を用いたヒトゲノムの実験的な注釈.

D D Shoemaker1, E E Schadt, C D Armour

  • 1Rosetta Inpharmatics, Inc., Kirkland, Washington 98034, USA.

Nature
|March 10, 2001
PubMed
まとめ
この要約は機械生成です。

実験的検証は,正確なゲノムアノテーションに不可欠です. 新しい配列ベースの方法は,遺伝子予測を洗練し,さまざまな条件でmRNAスプレイスの変種を特定します.

さらに関連する動画

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 6, 2008

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関連する実験動画

Last Updated: Jan 8, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

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Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform
13:14

Global Gene Expression Analysis Using a Zebrafish Oligonucleotide Microarray Platform

Published on: August 10, 2009

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Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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科学分野:

  • ゲノミクスゲノミクスとは
  • 分子生物学は分子生物学である.
  • バイオインフォマティックス

背景:

  • ゲノムシーケンシングは遺伝子カタログを生成しますが,計算上のアノテーションだけでは不十分です.
  • 実験的検証は,完全で正確な遺伝子および製品在庫のために不可欠です.

研究 の 目的:

  • 計算による遺伝子予測の検証と精錬のための実験的方法を開発し,適用する.
  • 全長トランスクリプトを定義し,mRNAスプライス変種を識別する.
  • 組織および疾患特有の遺伝子発現パターンを決定する.

主な方法:

  • インクジェットオリゴヌクレオチド合成によって製造されたエクソンとタイリング配列を使用した.
  • 完全長さのトランスクリプトを定義するためにエクソンの共同調節表現を適用します.
  • 69の条件下でヒト染色体22qと2つの条件下で全ヒトゲノムでこの技術をテストしました.

主要な成果:

  • 遺伝子予測の実験的ゲノムスケール検証の方法を実証した.
  • mRNAスプライス変種と組織/疾患特有の遺伝子発現の検出を可能にしました.
  • より正確な遺伝子カウントとトランスクリプトの定義を提供しました.

結論:

  • 実験的検証により,ゲノムアノテーションの正確性と信頼性が著しく向上します.
  • 開発された方法は,補完的なDNAクローニング戦略を改善します.
  • このアプローチは,複雑な疾患の研究において,潜在的な応用がある.