PASタンパク質のVIVIDは,光の入力を抑制し,ゲーティングを調節し,時計のリセットを調節する時計関連フィードバックループを定義します

C Heintzen¹, J J Loros, J C Dunlap

¹Department of Genetics, Dartmouth Medical School, Hanover, NH 03755, USA.

Cell

|March 10, 2001

まとめ

ニューロスポラのVVD遺伝子は,光への反応と昼夜リズムを調節する. それは早期抑制剤として作用し,光の適応とゲーティングに影響を与え,VVDを実証します.

科学分野:

シルカディアン生物学
分子遺伝学分子遺伝学
菌類学菌類学とは

背景:

昼間の時計は,日々の生物学的リズムを調節する.
光は,昼間の時計を同期させるための重要な環境的シグナルです.
Neurospora crassaは,昼夜リズムを研究するためのモデル生物として機能しています.

研究の目的:

ニューロスポラの光反応と昼夜回路におけるvvd遺伝子の役割を調査する.
PAS/LOVスーパーファミリーの新しいメンバーであるVVDタンパク質の機能を明らかにするために.
VVDが昼間の時計とどのように相互作用し,光の適応とゲーティングを調節するかを理解する.

主な方法:

異なる照明条件下でのvvdの遺伝子発現分析.
ニューロスパラのvvd変異株の構築と分析.
サーカディアンゲーティングと光刺激に対する相応答曲線の評価.

主要な成果:

vvdは,光反応に関与するPAS/LOVタンパク質をコードする.
VVDは,光誘発遺伝子の早期抑制剤として作用する.
vvdは時計制御であり,VVDは,frq遺伝子を含め,光応答の昼間ゲート制御を調節する.

結論:

VVDは,神経胞の昼間システム,特に光の適応と応答ゲーティングにおいて重要な役割を果たします.
核心時計の構成要素ではないが,VVDは,光信号を昼夜時計と統合するために不可欠である.
VVDは,光に対するフェーズリセット反応に影響を与え,昼夜系の強さに寄与します.

さらに関連する動画

09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

関連する概念動画

02:45

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...

02:19

Circadian Rhythms and Gene Regulation

01:24

Sleep-Wake Cycles

Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:

01:27

Time and frequency -Domain Interpretation of PI Control

Proportional-Integral (PI) controllers are essential in many control systems to improve stability and performance. They are commonly used in everyday devices like thermostats to enhance system damping and reduce steady-state error. When the zero in the controller's transfer function is optimally placed, the system benefits significantly in terms of stability and accuracy.
Acting as a low-pass filter, the PI controller slows the system's response and extends settling times. This requires careful...

01:21

Time and frequency -Domain Interpretation of Phase-lag Control

Phase-lag controllers are widely used in control systems to improve stability and reduce steady-state errors. A dimmer switch controlling the brightness of a light bulb serves as a practical example of phase-lag control, gradually adjusting the bulb's brightness. Mathematically, phase-lag control or low-pass filtering is represented when the factor 'a' is less than 1.
Phase-lag controllers do not place a pole at zero, but instead influence the steady-state error by amplifying any finite,...

関連する実験動画