関連する実験動画
Updated: Jun 19, 2026
10:10
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Published on: September 20, 2014
EGF受容体とNotchシグナリングは,Eyeless/Pax6の上流に作用し,目の仕様を制御する
1Department of Cell Biology, Emory University School of Medicine, 1648 Pierce Drive, Atlanta, GA 30322, USA.
Cell
|March 21, 2001
まとめ
ドロソフィラの眼の発達に対するマスターコントロール遺伝子は,早期に共発現しません. 代わりに,EGF受容体とNotchの信号伝達経路は,幼虫の第2段階の眼の特異化を開始します.
科学分野:
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- ドロソフィラの複合眼は,Eyeless/Pax6を含む7つの核因子の作用によって発達し,マスターコントロールタンパク質と呼ばれています.
- これらの要因は,外視的に発現すると目の発達を誘発し,変異すると目の損失を引き起こすことが知られている.
- 以前の理解によると,これらの遺伝子は胚の推定眼で共発現していた.
研究 の 目的:
- ドロソフィラの目の特異性遺伝子の正確なタイミングと細胞共同発現を調査する.
- 眼の発達に関与するマスター制御遺伝子の上流調節体を特定する.
- ドロソフィラの発達中に目の特異化が本当に起こる時期を判断する.
主な方法:
- 遺伝子発現パターンの遺伝子分析.
- EGFレセプターとノッチ信号伝達経路の役割を調査する.
- 重要な発達遺伝子の機能喪失と子宮外発現に関する研究.
主要な成果:
- いくつかの重要な眼の特異性遺伝子は,同一の胚細胞または推定眼で共発現していません.
- 皮膜成長因子 (EGF) 受容体とNotchシグナル伝達経路は,目の特異性遺伝子の上流で機能する.
- 眼の特異性は,胚の発達期ではなく,第2の幼虫期に発生することが示されています.
結論:
- ドロソフィラの眼の特異化は,以前考えられていたよりも遅いプロセスである.
- EGF受容体とNotch信号伝達経路は,目の発達を開始する上で重要なホメオティックな役割を果たします.
- 目特異遺伝子の空間的および時間的発現パターンは,当初提案されたマスターコントロールモデルよりも複雑です.
関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:22Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...