WNT信号は,FGF依存の四肢開始と,鶏の胚におけるAER誘導を制御する

Y Kawakami¹, J Capdevila, D Büscher

¹The Salk Institute for Biological Studies, Gene Expression Laboratory, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

Cell

|April 6, 2001

まとめ

3つのWNTシグナル伝達経路は,線維細胞成長因子 (FGF) 信号伝達を調節し,脊椎動物胚における四肢の発達とアピカル外皮 (AER) の形成を制御する.

科学分野:

発達生物学発達生物学について
分子生物学は分子生物学である.
遺伝学遺伝学とは

背景:

繊維細胞成長因子 (FGFs),特にFGF-8とFGF-10は,四肢の開始とアピカル外皮脊 (AER) の発達に不可欠です.
FGFシグナリングのアップストリームレギュレータを理解することは,胚の肢体のパターンを理解するために不可欠です.

研究の目的:

FGF-8/FGF-10の規制ループの調節におけるWNTシグナル伝達経路の役割を調査する.
手足の開始とAER誘導に関与する特定のWNT要因を特定する.

主な方法:

チキン胚におけるWNT因子の遺伝子発現分析.
WNT因子インプラントの際に遺伝子発現と子宮外肢形成を含む機能検査.

主要な成果:

Wnt-2bは,中間板および横板メソダームに発現し,Fgf-10および子宮外肢形成を誘発する.
Wnt-8cは,後肢領域のFgf-10を調節し,子宮外肢を誘発することもできる.
FGF-10は,手足の外皮のWnt-3a経由でFgf-8の発現を誘導する.

結論:

3つのWNTシグナル伝達経路は,β-カタニンによって媒介され,FGF-8/FGF-10ループの主要な調節体である.
これらのWNT信号は,脊椎動物の胚の発達中に,四肢の開始とAER誘導の両方をオーケストラします.

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