培養された線維芽細胞の生物学的時計の分子機構
K Yagita1, F Tamanini, G T van Der Horst
1Division of Molecular Brain Science, Department of Brain Sciences, Kobe University Graduate School of Medicine, Chuo-ku, Kobe 650-0017, Japan.
まとめ
哺乳類の周周周日波動振動器は,スプラキアスマティック核 (SCN) のようなもので,同一の分子機構を共有しています. 機能的な暗号染色体 (mCry) は,中央組織と周辺組織の両方で,これらの生物学的リズムに不可欠です.
科学分野:
- クロノバイオロジーはクロノバイオロジーを用います.
- 分子生物学は分子生物学である.
- 哺乳類生理学 哺乳類生理学について
背景:
- 哺乳類における中心的な昼間ペースメーカーは,上下神経核 (SCN) である.
- 周回組織にも昼間振動器が含まれているが,SCNに対するそれらの分子同一性は完全に理解されていない.
- クリプトクローム (mCry) は,哺乳類の昼間時計の構成要素として知られています.
研究 の 目的:
- 培養ファイブロブラストにおける周辺日経振動器の分子同一性を調査する.
- 哺乳類におけるSCN振動器とフィブロブラスト振動器を比較する.
- 周辺の昼夜リズムにおける暗号染色体 (mCry) の役割を決定する.
主な方法:
- mCry変異マウスから得られたワイルドタイプとmCry欠乏細胞系を使用した.
- 培養した線維芽細胞における既知の時計遺伝子の時間表現プロフィールを分析した.
- 評価されたmRNAリズム,タンパク質の局所化,および昼夜振動の周期長.
主要な成果:
- 周辺線維芽細胞振動器は,SCN振動器と同じ時間表現プロファイルを示しています.
- mRNAリズムフェッシング (例えば,Bmal1およびmPer遺伝子) とタンパク質核のエントリー遅延などの重要な特徴は保存されます.
- 振動はmCry欠乏細胞系には存在せず,mCryの重要な役割を確認した.
- mCRYタンパク質は,これらの周辺リズムの周期長さを制御することが判明しました.
結論:
- 哺乳類の線維芽細胞の周周周日波動器は,SCNペースメーカーと分子的に同一である.
- 機能的な暗号クローム (mCry) は,中央および周辺の昼間時計の両方の動作に不可欠です.
- これらの発見は,異なる哺乳類の組織にわたる昼間のタイミングの基礎となる保存された分子機構を強調しています.
関連する概念動画
Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
Biological Clocks and Seasonal Responses
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Cells Coordinate Growth and Proliferation
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Molecular Factors Affecting Cell Division
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...


