シーアにおけるタイムグラデーションは,空間グラデーションではないが,内皮細胞の増殖を刺激する
C R White1, M Haidekker, X Bao
1Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093-0412, USA.
Circulation
|May 23, 2001
まとめ
シェアストレスの時のタイムグラディエントは,ヒト内皮細胞 (HUVEC) の増殖を刺激する. 空間的グラデーションは,安定した切断ストレスの場合と比較して,HUVEC増殖に大きな影響を与えない.
科学分野:
- バイオメディカルエンジニアリング
- 細胞生物学 細胞生物学
- 流体力学 流体力学とは
背景:
- ヒト内皮細胞 (HUVECs) に及ぼすシーザーストレスの影響を調査する.
- 突然膨張フローチャンバー (SEFC) を使用して,時間対空間切断グラデーションを区別する.
研究 の 目的:
- HUVECの拡散における時間的および空間的なシーア・グラディエントの異なる役割を決定する.
- 流体切断に対する内皮細胞の反応のメカニズムを解明する.
主な方法:
- シェア曝露を制御するために突然膨張フロー室 (SEFC) を利用しました.
- 準確なタイムグラデント操作のために,平行プレートフロー室を使用した.
- さまざまなシール条件下 (突然発症,急増発症,安定したシール) で測定されたHUVEC増殖率.
主要な成果:
- ランパードフローの発生は,HUVECの有意な増殖を示さなかった.
- SEFCにおける突然の流れの発生は,再結合ゾーンにおけるHUVECの拡散を105%増加させた.
- ショアストレスの短い衝動はHUVECの増殖を大幅に増加させ,安定したショアはそれを抑制しました.
結論:
- シャーストレスの時のタイムグラディエントは,内皮細胞増殖の強力な刺激因子である.
- 空間切断グラデーションは,安定した切断効果を超えて,HUVECの増殖に独特な影響を与えない.
- シーア・グラデーションのダイナミクスを理解することは,内皮細胞機能と血管の健康にとって極めて重要です.
キーワード:
非プログラム的なものです.
関連する概念動画
01:12Renewal of Skin Epidermal Stem Cells
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular cells,...
01:06Multipotency and Niche of Bulge Stem Cell
A hair follicle or HF is a small part of the skin that produces the hair shaft. Paul Gerson Unna was the first to observe a bulge in the human hair follicle's outer root sheath (ORS). The bulge is present between the sebaceous gland and the arrector pili muscle and is the niche for hair follicle stem cells (HFSCs). The bulge is also a niche for melanocyte stem cells, and their loss results in graying of hair. The HFSCs express Sox9 and Lhx2, which help them maintain stemness and prevent...
01:24Cells of the Epidermis
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum.
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
01:11Papillary Dermis
Dermis
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
The dermis might be considered the "core" of the integumentary system, as distinct from the epidermis and hypodermis. It contains blood and lymph vessels, nerves, and other structures, such as hair follicles and sweat glands. The dermis is made of two layers of connective tissue that comprise an interconnected mesh of elastin and collagenous fibers, produced by fibroblasts.
Papillary Layer
The papillary layer is made of loose, areolar connective tissue, which means the collagen and...
01:16Accessory Structures of the Skin: Hair and Hair Follicles
Hair and hair follicles are integral components of the integumentary system. Hair is a filamentous structure composed mainly of a protein called keratin. It is found on the surface of the skin throughout the body, except for areas such as the palms of the hands and soles of the feet.
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
Hair is a keratinous filament growing out of the epidermis. It is primarily made of dead, keratinized cells. Hair strands originate at the epidermal penetration called the hair follicle. The hair shaft is the part...
01:20Accessory Structures of the Skin: Hair Growth and Types
Hair growth begins with the production of keratinocytes by the basal cells of the hair bulb. As new cells are deposited at the hair bulb, the hair shaft is pushed through the follicle toward the surface. Keratinization is completed as the cells are pushed to the skin surface to form the shaft of hair that is externally visible. The external hair is completely dead and composed entirely of keratin. Hair can be cut or shaven without damaging the hair structure because the cut is superficial. Most...