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設計された移行金属複合体を用いてタンパク質の表面認識

M A Fazal1, B C Roy, S Sun

  • 1Department of Chemistry, North Dakota State University, Fargo, ND 58105, USA.

Journal of the American Chemical Society
|June 28, 2001
PubMed
まとめ

研究者は,金属複合体を設計し,表面ヒスティジンのパターンをマッチすることで,炭酸アンヒドラゼのようなタンパク質に特異的に結合するようにしました. このタンパク質認識方法は,浄化,ターゲティング,センシングのための新しい方法を提供します.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...