関連する実験動画
Updated: Apr 29, 2026

10:06
Phagocytosis Assay for Apoptotic Cells in Drosophila Embryos
Published on: August 3, 2017
8.9K
ファゴサイトーシスは,C. elegansのプログラム細胞死を促進する.
P W Reddien1, S Cameron, H R Horvitz
1Howard Hughes Medical Institute, Department of Biology, 68-425, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA.
Nature
|July 13, 2001
まとめ
エングルフメント遺伝子は,C. elegans. のプログラム細胞死を促進する. これらの遺伝子の変異により,細胞が生き残り,分化することが可能になり,細胞死亡の完了を確実にする上で,吸収が重要な役割を果たしていることが明らかになりました.
科学分野:
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学とは
- 遺伝学 遺伝学とは
背景:
- C. elegansのプログラム細胞死 (PCD) は,キラー遺伝子 (egl-1, ced-4, ced-3) とエンゴルフメント遺伝子 (ced-1, ced-2, ced-5, ced-6, ced-7, ced-10, ced-12) を含む.
- PCD経路における吸収の正確な役割は,まだ完全に理解されていません.
研究 の 目的:
- プログラム細胞死を促進するエンゴルフメント遺伝子の役割を調査する.
- 包み込みが細胞死亡の完了に積極的に寄与するかどうかを判断する.
主な方法:
- キラー遺伝子の部分的な機能喪失を有するCayenorhabditis elegans変異体の分析.
- 特定の包み込み遺伝子を欠いている突然変異体の細胞生存と分化に関する評価.
- 遺伝子発現救済実験は,細胞を包み込む中で ced-1 を使用しています.
主要な成果:
- 飲み込み遺伝子の変異は,部分キラー遺伝子の欠陥を有する動物における細胞生存の頻度を高めます.
- エングルフメント遺伝子の突然変異は,死に定められた細胞の生存と分化につながる.
- 吸収細胞における ced-1 の発現は,Ced-1 ミュータントの細胞破壊欠陥を救済し,吸収細胞内で吸収遺伝子が作用することを示しています.
結論:
- 飲み込みは,プログラムされた細胞死を積極的に促進し,プロセスの完了を保証します.
- 吸収細胞は,PCDに開始された細胞の回復を防ぐ上で重要な役割を果たします.
- これは,CED-3カスパース媒介細胞死が不可逆であることを保証する細胞を包み込むモデルを示唆しています.
関連する概念動画
Overview of Cell Death
7.6K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.6K
Apoptosis
12.0K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.0K
Caspases
8.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
8.6K
Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K
Autophagic Cell Death
3.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.3K
Cellular Injury V: Apoptosis and Autophagy
87
Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
87

