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関連する概念動画

The DNA Helix01:16

The DNA Helix

Overview
The DNA Helix01:16

The DNA Helix

Overview
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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関連する実験動画

Updated: Jun 21, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

DNAオリゴマーの配列構造関係: 計算によるアプローチ

M J Packer1, C A Hunter

  • 1Contribution from the Krebs Institute for Biomolecular Science, Department of Chemistry, University of Sheffield, Sheffield, S3 7HF England. m.j.packer@shef.ac.uk

Journal of the American Chemical Society
|July 27, 2001
PubMed
まとめ

新しいDNA構造モデルは,塩基の積み重ねを分析することによって,オリゴーマー構成を正確に予測します. これは,ほとんどのDNAサンプルにおける実験データと一致する配列依存構造と低エネルギー状態を識別します.

科学分野:

  • 計算生物学とは,計算生物学である.
  • 構造生物学 構造生物学とは
  • バイオフィジックス 生物物理学

背景:

  • DNAの構造を予測することは,その機能を理解するために極めて重要です.
  • 配列に依存するDNA構成の変異 (A-DNAとB-DNA) は複雑である.
  • 実験データは,構造モデルのための貴重なベンチマークを提供します.

研究 の 目的:

  • DNAオリゴーマー構造を予測するための集団変数モデルを開発および検証.
  • 塩基堆積が配列依存のDNA構造に及ぼす影響を調査する.
  • モデル予測を高解像度の結晶構造と比較する.

主な方法:

  • すべての原子の塩基対と経験的バックボーンを持つ集合変数モデルを使用した.
  • 遺伝子アルゴリズムとグリッド検索を使用して,グローバルとローカルの最小エネルギー構造を見つけました.
  • A-およびB-DNAオリゴマーのロールとトウィストパラメータの傾向を分析した.

主要な成果:

  • 多様なDNAオリゴマーの5度以内のロールとツイストの実験的傾向を再現した.
  • 局所最小値の数がシーケンス依存度が高いことがわかりました.

さらに関連する動画

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

関連する実験動画

Last Updated: Jun 21, 2026

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

  • 30のオリゴマーのうち24の実験段階パラメータに一致する低エネルギーローカル最小値が特定されました.

    • 結論:

    • このモデルはDNAオリゴーマー構成をうまく予測し,塩基堆積の重要性を強調しています.
    • シーケンス固有の構造的変化と複数の低エネルギー状態の存在が観察されました.
    • 不一致は,結晶包装が固体DNA構成に及ぼす影響を示唆している.