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ヒストンライシンメチル化と,鶏のベータ・グロービン位置における発達変化の相関関係

M D Litt¹, M Simpson, M Gaszner

¹Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0540, USA.

Science (New York, N.Y.)

|August 11, 2001

まとめ

ヒストンのメチル化パターン,特にライシン9とライシン4は,赤血球の発達中の遺伝子活動と関連しています. リシン9メチル化は遺伝子静止と相関し,リシン4メチル化は活性遺伝子と関連している.

科学分野:

エピジェネティクスエピジェネティクス
分子生物学は分子生物学である.
遺伝子規制遺伝子規制

背景:

メチル化などのヒストンの改変は,遺伝子発現を制御するために重要である.
H3K9meとH3K4meのような特定のヒストンのメチル化マークは,異なる染色体状態と転写結果と関連しています.

研究の目的:

鶏のベータ・グロービン・ロカスにおけるエリトロポエーシス中のヒストンH3ライシン9 (H3K9me) とライシン4 (H3K4me) メチル化のパターンを調査する.
グロービン遺伝子発現の調節におけるH3K9me,H3K4me,ヒストンアセチル化の関係を理解する.

主な方法:

クロマチン免疫プレシピテーション (ChIP) を使用して,ヒストンH3 (H3K9me2) に対するディメチルライシン9を検出しました.
分析は,エリソポエシス中に鶏のベータ・グロービン・ロキュスの53キロベース領域にわたって行われました.

主要な成果:

H3K9me2は,β-グロービンの位置全体でH3アセチル化と逆相関を示した.
H3K9me2は,凝縮クロマチンの領域と,発達的に静止されたグロービンの遺伝子で濃縮された.
対照的にH3K4meはH3アセチル化と正の相関関係にある.

結論:

この研究は,単離剤が凝縮クロマチンを横に並べて,グロービン遺伝子を静止から保護するメカニズムを提案しています.
異なるヒストンのメチル化パターン (H3K9me vs. H3K4me) は,エリトポエーシス中に活性および非活性クロマチンの状態を区分する.

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